Strube Oliver I, Büngeler Anne, Bremser Wolfgang
†University of Paderborn, Department of Chemistry - Biobased and Bioinspired Materials, Warburger Str. 100, D-33098 Paderborn, Germany.
‡University of Paderborn, Department of Chemistry - Coating Materials and Polymers, Warburger Str. 100, D-33098 Paderborn, Germany.
Biomacromolecules. 2015 May 11;16(5):1608-13. doi: 10.1021/acs.biomac.5b00187. Epub 2015 Apr 9.
A method for in situ formation and controlled deposition of eumelanin nanoparticles is presented. The particles are built up by enzymatic reaction of l-DOPA with tyrosinase. The enzyme is tethered onto the support surface to get site-specific deposition of eumelanin. Due to the immediate deposition, the particles are monodisperse, with diameters of about 30-60 nm. Up to now, eumelanin particles have only been observed with sizes of about 200 nm. Deposition of those particles is site-specific on the areas where enzyme is present and results in different kinds of patterns on the support surface, including versatile monolayer structures.
本文提出了一种原位形成和可控沉积真黑素纳米颗粒的方法。这些颗粒通过左旋多巴与酪氨酸酶的酶促反应形成。该酶被固定在载体表面，以实现真黑素的位点特异性沉积。由于即时沉积，这些颗粒是单分散的，直径约为30-60纳米。到目前为止，仅观察到尺寸约为200纳米的真黑素颗粒。这些颗粒在位点特异性地沉积在存在酶的区域，并在载体表面形成不同类型的图案，包括多种单层结构。
