Gruhn Nijuscha, Seidl Michael F, Halawa Mhyeddeen, Heyl Alexander
a Institute of Biology/Applied Genetics; Dahlem Centre of Plant Sciences ; Freie Universität ; Berlin , Germany.
Plant Signal Behav. 2015;10(2):e984512. doi: 10.4161/21659087.2014.984512.
Cytokinins represent a group of plant hormones that have been shown to be essential for plant growth and development. A recent large-scale phylogenetic analysis of components of the cytokinin signal transduction pathway revealed, among other findings, the existence of a second, previously unknown subfamily of cytokinin receptors. Here we report that the cytokinin binding domains of the members of the 2 subfamilies contain residues that are highly conserved in either or in both subfamilies. Experiments using fluorescence microscopy hint at an ER and a plasma membrane localization for 2 members of the newly identified subfamily. These data provide new insights in the conservation of sequence and localization properties among the 2 subfamilies.
细胞分裂素是一类植物激素，已被证明对植物生长和发育至关重要。最近对细胞分裂素信号转导途径成分进行的大规模系统发育分析揭示了诸多发现，其中包括存在第二个先前未知的细胞分裂素受体亚家族。在此我们报告，这两个亚家族成员的细胞分裂素结合结构域含有在其中一个亚家族或两个亚家族中都高度保守的残基。使用荧光显微镜进行的实验表明，新鉴定亚家族的两个成员定位于内质网和质膜。这些数据为这两个亚家族之间序列和定位特性的保守性提供了新的见解。
