Raeburn Jaclyn, Chen Lin, Awhida Salmah, Deller Robert C, Vatish Manu, Gibson Matthew I, Adams Dave J
Department of Chemistry, University of Liverpool, Crown Street, Liverpool, L69 7ZD, UK.
Soft Matter. 2015 May 14;11(18):3706-13. doi: 10.1039/c5sm00456j.
A series of fluorescent probes, including a number of molecular rotors, have been used to follow the self-assembly of dipeptide-based low molecular weight gelators. We show that these probes can be used to gain an insight into the assembly process. Thioflavin T, a commonly used stain for β-sheets, appears to act as a molecular rotor in these gelling systems, with the fluorescence data closely matching that of other rotors. The molecular rotor was incorporated into an assay system with glucose oxidase to enable glucose-concentration specific gelation and hence generating a fluorescent output. Applying this system to urine from patients with various levels of glycosuria (a symptom of diabetes), it was found to provide excellent correlation with different clinical assessments of diabetes. This demonstrates a new concept in gelation-linked biosensing for a real clinical problem.
一系列荧光探针，包括许多分子转子，已被用于追踪基于二肽的低分子量凝胶剂的自组装过程。我们表明，这些探针可用于深入了解组装过程。硫黄素T是一种常用于检测β-折叠的染料，在这些凝胶体系中似乎起到了分子转子的作用，其荧光数据与其他转子的数据密切匹配。将该分子转子整合到含有葡萄糖氧化酶的检测系统中，以实现特定葡萄糖浓度下的凝胶化，并因此产生荧光输出。将该系统应用于不同糖尿水平（糖尿病的一种症状）患者的尿液中，发现它与糖尿病的不同临床评估具有良好的相关性。这证明了凝胶化相关生物传感在解决实际临床问题方面的一个新概念。
