Mayasari N, de Vries Reilingh G, Nieuwland M G B, Remmelink G J, Parmentier H K, Kemp B, van Knegsel A T M
Adaptation Physiology Group, Department of Animal Science, Wageningen University, PO Box 338, 6700 AH Wageningen, the Netherlands; Faculty of Animal Husbandry, Universitas Padjadjaran, 3636, Bandung, Indonesia.
Adaptation Physiology Group, Department of Animal Science, Wageningen University, PO Box 338, 6700 AH Wageningen, the Netherlands.
J Dairy Sci. 2015 Jun;98(6):3969-79. doi: 10.3168/jds.2014-8753. Epub 2015 Mar 28.
The objective was to study the effect of dry period length in dairy cows on immunoglobulin content and natural antibodies (NAb) titers in colostrum, growth, and plasma natural and specific antibody titers in plasma of calves. Holstein-Friesian dairy cows (n=167) were randomly assigned to 3 dry period lengths (0, 30, or 60 d). Colostrum production, concentration of colostrum IgG and IgM, and titers of NAb (isotypes IgG and IgM) binding keyhole limpet hemocyanin (KLH) and human serum albumin (HuSA) in colostrum were measured. Female calves were immunized with both KLH and HuSA at wk 6 and 10 of life. Titers of NAb and specific antibody (SpAb) for isotypes IgG, IgM, and total immunoglobulin (IgT) binding KLH or HuSA were determined in plasma of female calves. Primary and secondary antibody responses to KLH or HuSA from wk 6 and 10 were expressed as the increase in antibody titers to wk 10 and 11 of life after primary and secondary challenges, respectively. Pregnancy length for cows with a 0-d dry period was 3d shorter compared with cows with a 30- or 60-d dry period. Birth weight of calves from cows with a 0-d dry period was lower compared with calves from cows with a 30-d dry period. Growth of calves until 12 wk of life was not affected by dry period length. Colostrum production and IgG and IgM concentration in colostrum were lower for cows with a 0-d dry period than a 60-d dry period. Natural IgG and IgM titers binding KLH or HuSA were lower in colostrum from cows with a 0-d dry period compared with cows with a 60-d dry period. Natural antibody titers (IgG, IgM, and IgT) binding KLH or HuSA in plasma were lower during the first 2 wk of life for calves from cows with a 0-d dry period compared with calves from cows with a 30- or 60-d dry period. After primary and secondary immunization of calves with KLH and HuSA, SpAb titers of calves were not affected by dry period length. After secondary immunization, the response of IgG and IgT binding KLH was higher in plasma of calves from cows with a 0-d dry period. The results of this study demonstrate that, although omission of the dry period of dairy cows leads to lower plasma NAb titers in calves during the first 2 wk of life, SpAb titers in calves were not affected and even the secondary antibody responses were enhanced compared with calves from cows with a 30- or 60-d dry period.
本研究的目的是探讨奶牛干奶期长短对初乳中免疫球蛋白含量和天然抗体（NAb）滴度、犊牛生长以及血浆中天然和特异性抗体滴度的影响。将167头荷斯坦 - 弗里生奶牛随机分为3个干奶期长度组（0、30或60天）。测定初乳产量、初乳中IgG和IgM的浓度以及初乳中结合钥孔血蓝蛋白（KLH）和人血清白蛋白（HuSA）的NAb（IgG和IgM亚型）滴度。雌性犊牛在出生后第6周和第10周分别用KLH和HuSA进行免疫。测定雌性犊牛血浆中结合KLH或HuSA的IgG、IgM和总免疫球蛋白（IgT）亚型的NAb和特异性抗体（SpAb）滴度。将出生后第6周和第10周对KLH或HuSA的初次和二次抗体反应分别表示为初次和二次免疫后至出生后第10周和第11周抗体滴度的增加。与干奶期为30天或60天的奶牛相比，干奶期为0天的奶牛妊娠期短3天。与干奶期为30天的奶牛所产犊牛相比，干奶期为0天的奶牛所产犊牛出生体重较低。犊牛至12周龄的生长不受干奶期长短的影响。干奶期为0天的奶牛初乳产量以及初乳中IgG和IgM浓度低于干奶期为60天的奶牛。与干奶期为60天的奶牛相比，干奶期为0天的奶牛初乳中结合KLH或HuSA的天然IgG和IgM滴度较低。与干奶期为30天或60天的奶牛所产犊牛相比，干奶期为0天的奶牛所产犊牛出生后前2周血浆中结合KLH或HuSA的天然抗体滴度（IgG、IgM和IgT）较低。在用KLH和HuSA对犊牛进行初次和二次免疫后，犊牛的SpAb滴度不受干奶期长短的影响。二次免疫后，干奶期为0天的奶牛所产犊牛血浆中结合KLH的IgG和IgT反应较高。本研究结果表明，虽然省略奶牛干奶期会导致犊牛出生后前2周血浆中NAb滴度降低，但犊牛的SpAb滴度不受影响，与干奶期为30天或60天的奶牛所产犊牛相比，甚至二次抗体反应增强。
