Mayasari N, Van Knegsel A T M, de Vries Reilingh G, Kemp B, Parmentier H K
Adaptation Physiology Group, Department of Animal Science, Wageningen University, P.O. Box 338, 6700 AH Wageningen, the Netherlands; Faculty of Animal Husbandry, Universitas Padjadjaran, 45363 Bandung, Indonesia.
Adaptation Physiology Group, Department of Animal Science, Wageningen University, P.O. Box 338, 6700 AH Wageningen, the Netherlands.
Vet Immunol Immunopathol. 2016 Oct 1;178:70-8. doi: 10.1016/j.vetimm.2016.07.001. Epub 2016 Jul 2.
Natural autoantibodies (NAAb) have a role in maintaining physiological homeostasis and prevention of infections, and have been found in mammalian species tested so far. Albeit NAAb levels rise with age, little is known about the origin, function, regulation and initiation of NAAb in young animals. The present study addressed the presence of IgM and IgG NAAb binding glutamate dehydrogenase (GD), carbonic anhydrase (CA), myosin (MYO) and transferrin (TRANS) from before drinking colostrum until the first 12 weeks of life in plasma of female calves. In addition, NAAb to these four self-antigens were also measured in colostrum and in plasma of their mothers during three weeks before calving. Titers of NAAb binding GD, CA, MYO and TRANS were detected in plasma of cows before calving, in colostrum, and in plasma of calves before and after drinking of colostrum. Levels of NAAb in colostrum were positively related with levels of NAAb in plasma of cows. Before colostrum intake, levels of NAAb in plasma of calves were not related with levels of NAAb in plasma of their mother but were influenced by parity of their mother. After colostrum intake, levels of NAAb in plasma of calves in the first week of life were positively related with levels of NAAb in colostrum. Low NAAb levels in colostrum were related with low NAAb in plasma of calves in the first week of life, but after two weeks of life the relation between colostrum and plasma of calves was absent. In conclusion, NAAb are already present in the unborn calf, and levels of neonatal NAAb during the early weeks of life are affected by levels of maternal NAAb obtained via colostrum.
天然自身抗体（NAAb）在维持生理稳态和预防感染方面发挥着作用，并且在目前已检测的哺乳动物物种中均有发现。尽管NAAb水平会随着年龄增长而升高，但对于幼龄动物体内NAAb的起源、功能、调节及起始情况却知之甚少。本研究探讨了雌性犊牛从出生前直至出生后12周血浆中免疫球蛋白M（IgM）和免疫球蛋白G（IgG）型NAAb与谷氨酸脱氢酶（GD）、碳酸酐酶（CA）、肌球蛋白（MYO）和转铁蛋白（TRANS）的结合情况。此外，还检测了犊牛出生前3周其母亲初乳及血浆中针对这四种自身抗原的NAAb。在母牛产犊前血浆、初乳以及犊牛饮用初乳前后的血浆中均检测到了与GD、CA、MYO和TRANS结合的NAAb滴度。初乳中NAAb水平与母牛血浆中NAAb水平呈正相关。在摄入初乳前，犊牛血浆中NAAb水平与其母亲血浆中NAAb水平无关，但受其母亲胎次的影响。摄入初乳后，出生后第一周犊牛血浆中NAAb水平与初乳中NAAb水平呈正相关。初乳中NAAb水平较低与出生后第一周犊牛血浆中NAAb水平较低有关，但出生两周后犊牛初乳与血浆之间的这种关系消失。总之，未出生的犊牛体内已存在NAAb，并且生命早期几周新生NAAb的水平受通过初乳获得的母体NAAb水平影响。
