Yang Fan, Song Yanqing, Zhang Sixi, Zhou Wei
Department of Pediatric Surgery, the First Hospital of Jilin University, Changchun, 130021, China.
Department of Pharmacy, the First Hospital of Jilin University, Changchun, 130021, China.
Biomed Chromatogr. 2015 Oct;29(10):1619-22. doi: 10.1002/bmc.3464. Epub 2015 Mar 31.
A simple, sensitive and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the determination of calceorioside B (CLB) in rat plasma. Detection was performed on a Thermo Scientific Hypersil Gold chromatography column using isocratic elution with a mobile phase of methanol-5 m m ammonium acetate-formic acid (70:30:0.1, v/v/v). Mass spectrometry was performed in selection reaction monitoring mode using a positive electrospray ionization interface. Good linearity was found for CLB in plasma in the linear range of 1.00-500 ng/mL (r > 0.9960). The validated method was successfully applied to the pharmacokinetic study of CLB in rats.
建立了一种简单、灵敏、快速的液相色谱-串联质谱（LC-MS/MS）法，并对其进行了验证，用于测定大鼠血浆中的钙皮苷B（CLB）。采用Thermo Scientific Hypersil Gold色谱柱，以甲醇-5 mM醋酸铵-甲酸（70:30:0.1，v/v/v）为流动相进行等度洗脱，进行检测。采用正电喷雾电离接口，在选择反应监测模式下进行质谱分析。血浆中CLB在1.00-500 ng/mL线性范围内具有良好的线性（r>0.9960）。该验证方法成功应用于CLB在大鼠体内的药代动力学研究。
