Jia Ganchu, Liang Jian, Xie Jun, Wang Jun, Bao Zhenmin, Xie Liping, Zhang Rongqing
Institute of Marine Biotechnology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Key Laboratory of Marine Genetics and Breeding (MGB), Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao, China.
Comp Biochem Physiol B Biochem Mol Biol. 2015 Jul;185:34-41. doi: 10.1016/j.cbpb.2015.03.004. Epub 2015 Mar 28.
Matrix proteins play an important role in biomineralization by mollusks. In this study, we cloned and characterized an acidic protein (pI=3.36) homolog of cfMSP-1 that is highly expressed in the mantle transcriptome of the scallop Chlamys farreri. RT-PCR and in situ hybridization showed that cfMSP-1 is specifically expressed in the outer fold of the mantle edge and pallial part. The expression level of cfMSP-1 remarkably increased and then reduced gradually to a value that is ~2-fold higher than basal levels after shell notching. Knock-down expression of cfMSP-1 in adults via dsRNA injection gave a disordered folia surface. Both shell notching and RNAi experiments indicated that cfMSP-1 plays an essential role in the formation of the folia of C. farreri.
基质蛋白在软体动物的生物矿化过程中发挥着重要作用。在本研究中，我们克隆并鉴定了一种与cfMSP-1同源的酸性蛋白（pI = 3.36），该蛋白在栉孔扇贝外套膜转录组中高表达。RT-PCR和原位杂交表明，cfMSP-1特异性表达于外套膜边缘的外褶和鳃区。在贝壳刻痕后，cfMSP-1的表达水平显著升高，然后逐渐降低至比基础水平高约2倍的值。通过注射dsRNA在成体中敲低cfMSP-1的表达导致叶状结构表面紊乱。贝壳刻痕和RNA干扰实验均表明，cfMSP-1在栉孔扇贝叶状结构的形成中起关键作用。
