Gou Yujun, Zhang Feng, Zhu Xiaoyan, Li Xiangqian
Faculty of Life Science and Chemical Engineering, HuaiYin Institute of Technology, Huaian 223003, The People's Republic of China.
Enzyme and Biomaterials Center, Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaian, 223003, The People's Republic of China.
IET Nanobiotechnol. 2015 Apr;9(2):53-7. doi: 10.1049/iet-nbt.2014.0005.
Sphingomonas is a novel and abundant microbial resource for biodegradation of aromatic compounds. It has great potential in environment protection and industrial production. The use of microorganisms for the synthesis of nanoparticles is in the limelight of modern nanotechnology, since it is cost effective, non-toxic and friendly to the ever-overwhelmed environment. In this paper, the biosynthesis of silver nanoparticles (AgNPs) using Sphingomonas paucimobilis sp. BDS1 under ambient conditions was investigated for the first time. Biosynthesised AgNPs were characterised with powder ultraviolet-visible spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscopy and energy dispersive X-ray spectroscopy. The overall results revealed that well-dispersed face centred cubic spherical AgNPs in the range of 50-80 nm were produced on the surface of Sphingomonas paucimobilis sp. BDS1, after challenging pure wet biomass with silver nitrate solution. This suggests that the capture of silver ions may be a complex process of physical and chemical adsorption and the proteins on the surface of the bacteria may play the role of reduction and stabilising agent with regard to the result of FTIR.
鞘氨醇单胞菌是一种用于芳香族化合物生物降解的新型且丰富的微生物资源。它在环境保护和工业生产中具有巨大潜力。利用微生物合成纳米颗粒是现代纳米技术的研究热点，因为它具有成本效益、无毒且对日益不堪重负的环境友好。本文首次研究了在环境条件下利用少动鞘氨醇单胞菌BDS1菌株生物合成银纳米颗粒（AgNPs）。通过粉末紫外可见光谱、X射线衍射、傅里叶变换红外光谱（FTIR）、场发射扫描电子显微镜和能量色散X射线光谱对生物合成的AgNPs进行了表征。总体结果表明，在用硝酸银溶液处理纯湿生物质后，在少动鞘氨醇单胞菌BDS1菌株表面产生了尺寸在50 - 80 nm范围内、分散良好的面心立方球形AgNPs。这表明银离子的捕获可能是一个物理和化学吸附的复杂过程，并且就FTIR结果而言，细菌表面的蛋白质可能起到还原和稳定剂的作用。
