Li Yong, Tang Xiaoling, Song Wenshuang, Zhu Lina, Liu Xingang, Yan Xiaomin, Jin Chengzhi, Ren Qingguang
Center of Analysis and Measurement, Fudan University, Shanghai 200433, People's Republic of China.
IET Nanobiotechnol. 2015 Feb;9(1):19-26. doi: 10.1049/iet-nbt.2013.0062.
Extracellular and intracellular biosynthesis of silver nanoparticles (AgNPs) by Euglena gracilis (EG) strain and Euglena intermedia (EI) strain are reported in this study. The obtained nanoparticles showed an absorption peak approximates 420 nm in the UV-visible spectrum, corresponding to the plasmon resonance of AgNPs. According to the result of inductively coupled plasma-atomic emission spectrometer, the intakes of silver ions by EI and EG are roughly equal. The transmission electron microscope (TEM) analysis of the successful in vivo and in vitro synthesised AgNPs indicated the sizes, ranging from 6 to 24 nm and 15 to 60 nm in diameter, respectively, and a spherical-shaped polydispersal of the particles. The successful formation of AgNPs has been confirmed by energy dispersive X-ray analysis connected to the TEM. The Fourier transform infrared spectroscopy measurements reveal the presence of bioactive functional groups such as amines are found to be the capping and stabilising agents of nanoparticles. To our knowledge, this is the first report where two kinds of Euglena microalga were used as the potential source for in vivo and in vitro biosynthesis of AgNPs.
本研究报道了纤细裸藻（EG）菌株和中间裸藻（EI）菌株对银纳米颗粒（AgNPs）的胞外和胞内生物合成。所获得的纳米颗粒在紫外可见光谱中显示出一个约420nm的吸收峰，对应于AgNPs的等离子体共振。根据电感耦合等离子体原子发射光谱仪的结果，EI和EG对银离子的摄取量大致相等。对成功在体内和体外合成的AgNPs进行的透射电子显微镜（TEM）分析表明，其尺寸分别为直径6至24nm和15至60nm，且颗粒呈球形多分散。与TEM相连的能量色散X射线分析证实了AgNPs的成功形成。傅里叶变换红外光谱测量揭示了存在诸如胺等生物活性官能团，这些官能团被发现是纳米颗粒的封端和稳定剂。据我们所知，这是首次报道将两种裸藻微藻用作AgNPs体内和体外生物合成的潜在来源。
