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利用朱槿花瓣提取物合成、表征银纳米颗粒及其抗菌活性

Biosynthesis, characterisation and antimicrobial activity of silver nanoparticles using Hibiscus rosa-sinensis petals extracts.

作者信息

Nayak Debasis, Ashe Sarbani, Rauta Pradipta Ranjan, Nayak Bismita

机构信息

Immunology and Molecular Medicine Laboratory, Department of Life Science, National Institute of Technology, Rourkela, Odisha, India.

出版信息

IET Nanobiotechnol. 2015 Oct;9(5):288-93. doi: 10.1049/iet-nbt.2014.0047.

Abstract

Green synthesis of metallic nanoparticles has lured the world from the chemical and physical approaches owing to its rapid, non-hazardous and economic aspect of production mechanism. In this study, silver nanoparticles (AgNPs) were synthesised using petal extracts of Hibiscus rosa-sinensis. The AgNPs displayed characteristic surface plasmon resonance peak at around 421 nm having a mean particle size of 76.25±0.17 nm and carried a charge of -41±0.2 mV. The X-ray diffraction patterns displayed typical peaks of face centred cubic crystalline silver. The surface morphology was characterised by scanning electron microscopy and atomic force microscopy. Fourier transform infrared spectroscopy studies confirmed the surface modifications of the functional groups for the synthesis of AgNPs. Furthermore, the synthesised AgNPs displayed proficient antimicrobial activity against pathogenic strains of Vibrio cholerae, Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus.

摘要

由于金属纳米颗粒的绿色合成在生产机制上具有快速、无害和经济的特点,因此吸引了全世界摒弃化学和物理方法。在本研究中,使用朱槿花瓣提取物合成了银纳米颗粒(AgNPs)。AgNPs在约421nm处显示出特征性表面等离子体共振峰,平均粒径为76.25±0.17nm,电荷为-41±0.2mV。X射线衍射图谱显示出面心立方晶体银的典型峰。通过扫描电子显微镜和原子力显微镜对表面形态进行了表征。傅里叶变换红外光谱研究证实了用于合成AgNPs的官能团的表面修饰。此外,合成的AgNPs对霍乱弧菌、大肠杆菌、肺炎克雷伯菌和金黄色葡萄球菌的致病菌株表现出高效的抗菌活性。

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