Müller Premru Manica, Beović Bojana, Pokorn Marko, Cvitković Špik Vesna
Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloška 4, 1000, Ljubljana, Slovenia.
Department of Infectious Diseases, University Medical Centre, Ljubljana, Slovenia.
Wien Klin Wochenschr. 2015 Sep;127(17-18):691-5. doi: 10.1007/s00508-015-0721-y. Epub 2015 Apr 1.
To investigate epidemiology of invasive pneumococcal disease (IPD) in the central part of Slovenia in a population with no routine pneumococcal vaccination, we carried out serotyping of isolates by sequential multiplex polymerase chain reaction (PCR) and genotyping by repetitive sequence-based PCR (rep-PCR) and some by multilocus sequence typing. IPD was confirmed in 134 (26.5 %) of 510 acutely ill patients, either by a positive blood culture or real-time PCR (rt-PCR). In 94 patients, isolates were available for typing (24 from blood and 70 from nasopharynx). They belonged to 12 different serotypes; the most prevalent were 14 (27.6 % isolates), 9V, 3 (12.7 % each), 7F (9.5 %), 19A, and 1 (7.4 % each) followed by 4, 6A/B, 19F, 23F, 18C, and 33F. Genotyping yielded 34 rep-PCR genotypes and 13 subtypes; six were found in serotype 14, one in 9V, four each in 3, 19A, and 6A/B, three each in 7F and 1, and two each in 4, 19F, 23F, and 18C. Serotype 9V was the most homogenous and 14 and 19A were heterogenous and had two divergent clonal groups each. The most common genotypes belonged to virulent widespread clones, like ST162, ST9, ST15, ST156, ST191, and ST1377; however, sporadic clones were also observed.
为了调查在斯洛文尼亚中部未进行常规肺炎球菌疫苗接种人群中侵袭性肺炎球菌疾病（IPD）的流行病学情况，我们通过连续多重聚合酶链反应（PCR）对分离株进行血清分型，并通过基于重复序列的PCR（rep-PCR）进行基因分型，部分采用多位点序列分型。在510例急性病患者中，134例（26.5%）通过血培养阳性或实时PCR（rt-PCR）确诊为IPD。94例患者有可供分型的分离株（24例来自血液，70例来自鼻咽部）。它们属于12种不同血清型；最常见的是14型（占分离株的27.6%）、9V型、3型（各占12.7%）、7F型（9.5%）、19A型和1型（各占7.4%），其次是4型、6A/B型、19F型、23F型、18C型和33F型。基因分型产生了34种rep-PCR基因型和13种亚型；14型中有6种，9V型中有1种，3型、19A型和6A/B型各有4种，7F型和1型各有3种，4型、19F型、23F型和18C型各有2种。9V型血清型最为均一，14型和19A型血清型不均一，各有两个不同的克隆群。最常见的基因型属于毒性广泛传播的克隆，如ST162、ST9、ST15、ST156、ST191和ST1377；然而，也观察到了散发性克隆。
