Marvizon Juan Carlos, Walwyn Wendy, Minasyan Ani, Chen Wenling, Taylor Bradley K
Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California.
Veteran Affairs Greater Los Angeles Healthcare System, Los Angeles, California.
Curr Protoc Neurosci. 2015 Apr 1;71:9.50.1-9.50.14. doi: 10.1002/0471142301.ns0950s71.
Latent sensitization is a rodent model of chronic pain that reproduces both its episodic nature and its sensitivity to stress. It is triggered by a wide variety of injuries ranging from injection of inflammatory agents to nerve damage. It follows a characteristic time course in which a hyperalgesic phase is followed by a phase of remission. The hyperalgesic phase lasts between a few days to several months, depending on the triggering injury. Injection of μ-opioid receptor inverse agonists (e.g., naloxone or naltrexone) during the remission phase induces reinstatement of hyperalgesia. This indicates that the remission phase does not represent a return to the normal state, but rather an altered state in which hyperalgesia is masked by constitutive activity of opioid receptors. Importantly, stress also triggers reinstatement. Here we describe in detail procedures for inducing and following latent sensitization in its different phases in rats and mice.
潜伏致敏是一种慢性疼痛的啮齿动物模型，它再现了慢性疼痛的发作性本质及其对应激的敏感性。它由从注射炎性介质到神经损伤等各种各样的损伤引发。它遵循一个特征性的时间进程，其中痛觉过敏期之后是缓解期。痛觉过敏期持续数天至数月不等，这取决于引发损伤的类型。在缓解期注射μ-阿片受体反向激动剂（如纳洛酮或纳曲酮）会诱导痛觉过敏的恢复。这表明缓解期并不代表恢复到正常状态，而是一种阿片受体组成性活性掩盖了痛觉过敏的改变状态。重要的是，应激也会触发恢复。在此，我们详细描述在大鼠和小鼠中诱导和跟踪潜伏致敏不同阶段的程序。
