Mell Blair, Jala Venkatakrishna R, Mathew Anna V, Byun Jaeman, Waghulde Harshal, Zhang Youjie, Haribabu Bodduluri, Vijay-Kumar Matam, Pennathur Subramaniam, Joe Bina
Center for Hypertension and Personalized Medicine, Department of Physiology and Pharmacology, University of Toledo College of Medicine and Life Sciences, Toledo, Ohio;
James Graham Brown Cancer Center, Department of Microbiology and Immunology, University of Louisville, Louisville, Kentucky;
Physiol Genomics. 2015 Jun;47(6):187-97. doi: 10.1152/physiolgenomics.00136.2014. Epub 2015 Mar 31.
The gut microbiota plays a critical role in maintaining physiological homeostasis. This study was designed to evaluate whether gut microbial composition affects hypertension. 16S rRNA genes obtained from cecal samples of Dahl salt-sensitive (S) and Dahl salt-resistant (R) rats were sequenced. Bacteria of the phylum Bacteroidetes were higher in the S rats compared with the R rats. Furthermore, the family S24-7 of the phylum Bacteroidetes and the family Veillonellaceae of the phylum Firmicutes were higher in the S rats compared with the R rats. Analyses of the various phylogenetic groups of cecal microbiota revealed significant differences between S and R rats. Both strains were maintained on a high-salt diet, administered antibiotics for ablation of microbiota, transplanted with S or R rat cecal contents, and monitored for blood pressure (BP). Systolic BP of the R rats remained unaltered irrespective of S or R rat cecal transplantation. Surprisingly, compared with the S rats given S rat cecal content, systolic BP of the S rats given a single bolus of cecal content from R rats was consistently and significantly elevated during the rest of their life, and they had a shorter lifespan. A lower level of fecal bacteria of the family Veillonellaceae and increased plasma acetate and heptanoate were features associated with the increased BP observed in the S rats given R rat microbiota compared with the S rats given S rat microbiota. These data demonstrate a link between microbial content and BP regulation and, because the S and R rats differ in their genomic composition, provide the necessary basis to further examine the relationship between the host genome and microbiome in the context of BP regulation in the Dahl rats.
肠道微生物群在维持生理稳态中起着关键作用。本研究旨在评估肠道微生物组成是否会影响高血压。对从 Dahl 盐敏感（S）大鼠和 Dahl 盐抵抗（R）大鼠盲肠样本中获得的 16S rRNA 基因进行测序。与 R 大鼠相比，S 大鼠中拟杆菌门的细菌含量更高。此外，与 R 大鼠相比，S 大鼠中拟杆菌门的 S24 - 7 科和厚壁菌门的韦荣氏菌科含量更高。对盲肠微生物群的各种系统发育组分析显示，S 大鼠和 R 大鼠之间存在显著差异。两种品系的大鼠均维持高盐饮食，给予抗生素以消除微生物群，移植 S 或 R 大鼠的盲肠内容物，并监测血压（BP）。无论移植 S 大鼠还是 R 大鼠的盲肠内容物，R 大鼠的收缩压均保持不变。令人惊讶的是，与给予 S 大鼠盲肠内容物的 S 大鼠相比，给予单次大剂量 R 大鼠盲肠内容物的 S 大鼠在其剩余生命中收缩压持续且显著升高，并且寿命更短。与给予 S 大鼠微生物群的 S 大鼠相比，给予 R 大鼠微生物群的 S 大鼠中观察到的血压升高与韦荣氏菌科粪便细菌水平较低以及血浆乙酸盐和庚酸盐增加有关。这些数据证明了微生物含量与血压调节之间的联系，并且由于 S 大鼠和 R 大鼠在基因组组成上存在差异，为进一步研究 Dahl 大鼠血压调节背景下宿主基因组与微生物组之间的关系提供了必要依据。
