Yamamoto Hiroyuki, Kondo Kazuhiro, Tanaka Takayuki, Muramatsu Takahiko, Yoshida Hiroo, Imaizumi Akira, Nagao Kenji, Noguchi Yasushi, Miyano Hiroshi
Institute for Innovation, Ajinomoto Co., Inc., Kawasaki, Japan.
Institute for Innovation, Ajinomoto Co., Inc., Kawasaki, Japan
Ann Clin Biochem. 2016 May;53(Pt 3):357-64. doi: 10.1177/0004563215583360. Epub 2015 Mar 31.
Plasma amino acid concentrations vary with various diseases. Although reference intervals are useful in daily clinical practice, no reference intervals have been reported for plasma amino acids in a large Japanese population.
Reference individuals were selected from 7685 subjects examined with the Japanese Ningen Dock in 2008. A total of 1890 individuals were selected based on exclusion criteria, and the reference samples were selected after the outlier samples for each amino acid concentration were excluded. The lower limit of the reference intervals for the plasma amino acid concentrations was set at the 2.5th percentile and the upper limit at the 97.5th percentile.
By use of the nested analysis of variance, we analysed a large dataset of plasma samples and the effects of background factors (sex, age and body mass index [BMI]) on the plasma amino acid concentrations. Most amino acid concentrations were related to sex, especially those of branched-chained amino acid. The citrulline, glutamine, ornithine and lysine concentrations were related to age. The glutamate concentration was related to body mass index.
The concentrations of most amino acids are more strongly related to sex than to age or body mass index. Our results indicate that the reference intervals for plasma amino acid concentrations should be stratified by sex when the background factors of age and body mass index are considered.
血浆氨基酸浓度会因各种疾病而有所不同。虽然参考区间在日常临床实践中很有用，但在大量日本人群中尚未有血浆氨基酸参考区间的报道。
参考个体选自2008年接受日本国民健康体检的7685名受试者。根据排除标准共选取了1890名个体，并在排除每种氨基酸浓度的异常样本后选取参考样本。血浆氨基酸浓度参考区间的下限设定为第2.5百分位数，上限设定为第97.5百分位数。
通过使用方差嵌套分析，我们分析了一个庞大的血浆样本数据集以及背景因素（性别、年龄和体重指数[BMI]）对血浆氨基酸浓度的影响。大多数氨基酸浓度与性别有关，尤其是支链氨基酸的浓度。瓜氨酸、谷氨酰胺、鸟氨酸和赖氨酸浓度与年龄有关。谷氨酸浓度与体重指数有关。
大多数氨基酸的浓度与性别的相关性比与年龄或体重指数的相关性更强。我们的结果表明，在考虑年龄和体重指数等背景因素时，血浆氨基酸浓度的参考区间应按性别分层。
