Department of GCP, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, P.R. China.
Dalian Boyuan Medical Technology Co. Ltd, Dalian, P.R. China.
Bioanalysis. 2024;16(13):693-704. doi: 10.1080/17576180.2024.2387467. Epub 2024 Aug 19.
Branched-chain amino acids (BCAAs) and aromatic amino acids (AAAs) were suggested as potential biomarkers in liver disease. This study aimed to develop and validate a simple and rapid LC-MS/MS method to simultaneously measure serum BCAAs and AAAs levels in patients with liver injury, and further establish reference intervals of Chinese healthy adult populations. Samples were prepared by a one-step protein precipitation and analysis time was 4 min per run. The validation results showed good linearity (r >0.9969), satisfactory accuracy (94.44% - 107.75%) and precision (0.10% - 5.90%). This method proved to be suitable for high-throughput routine clinical use and could be a valuable adjunct diagnosis tool for liver injury and other clinical applications.
支链氨基酸 (BCAAs) 和芳香族氨基酸 (AAAs) 被认为是肝病潜在的生物标志物。本研究旨在开发和验证一种简单、快速的 LC-MS/MS 方法，以同时测量肝损伤患者血清中 BCAAs 和 AAAs 的水平，并进一步建立中国健康成年人的参考区间。样品采用一步法蛋白沉淀进行预处理，分析时间为每个运行 4 分钟。验证结果表明该方法具有良好的线性（r＞0.9969）、满意的准确度（94.44% - 107.75%）和精密度（0.10% - 5.90%）。该方法证明适用于高通量常规临床使用，可作为肝损伤和其他临床应用的有价值的辅助诊断工具。
