Watson J V
MRC Clinical Oncology Unit, Medical School, Cambridge, England.
Cytometry. 1989 Nov;10(6):681-8. doi: 10.1002/cyto.990100603.
A compact, solid, spherico-ellipsoidal chamber (SEC), which has approaching 4 pi ("all around") light collection, has been developed for flow cytometry. This was mounted onto the stage of a standard fluorescence photomicroscope, and the camera was replaced by a photomultiplier. Both components can be added or removed in minutes. The increased light collection efficiency of the SEC (about 85%) compared with about 4% from standard chambers enabled a fluorescence microscope with a 50 W mercury vapour lamp to "double" as a flow cytometer. The system was tested with microbeads and cells stained for DNA with ethidium bromide, and results were comparable to those obtained with our laser-based instrument.
一种紧凑、坚固的球椭球形腔室（SEC）已被开发用于流式细胞术，该腔室具有接近4π（“全方位”）的光收集能力。它被安装在标准荧光显微镜的载物台上，相机被光电倍增管取代。这两个组件都可以在几分钟内添加或移除。与标准腔室约4%的光收集效率相比，SEC的光收集效率提高到了约85%，这使得一台配备50W汞蒸气灯的荧光显微镜能够兼作流式细胞仪。该系统用微珠和用溴化乙锭染色的DNA细胞进行了测试，结果与我们基于激光的仪器所获得的结果相当。
