Zhang Xianwei, Jiang Aimin, Chen Mingtsao, Ockerman Herbert W, Chen Jiaojiao
College of Food Science, South China Agriculture University, Tianhe Road, Guangzhou, 510642 People's Republic of China.
Department of Bioindustry Technology, Da Yeh University, 168 University Rd. Dacum, Changhua, Taiwan.
J Food Sci Technol. 2015 Apr;52(4):2264-71. doi: 10.1007/s13197-013-1201-x. Epub 2013 Nov 8.
Changes in chemical composition, physical property and microstructure of pidan white treated with 4.5 % NaOH or 5.5 % KOH were monitored during pickling up to 4 weeks, and followed by aging for another 2 weeks. As the pickling and ageing times increased, moisture content of pidan white decreased and salt content increased for both (4.5 % NaOH and 5.5 % KOH) treatments (P < 0.05). Free alkalinity and pH of pidan white treated with 4.5 % NaOH increased as pickling proceeded, but decreased during ageing for both pickling treatments (P < 0.05). At week 4 of pickling, pidan white treated with 5.5 % KOH had higher hardness, cohesiveness, gumminess and chewiness than those treated with 4.5 % NaOH. After ageing, higher springiness, elastic modulus (G') and viscous modulus (G") were generally found in pidan white treated with 5.5 % KOH (P < 0.05). As the pickling time increased, lower L*, b* values and higher a* value were observed in pidan white from both treatments (P < 0.05). As visualized by scanning electron microscope, the aggregation of egg proteins took place in pidan white gels, irrespective of pickling treatments used. Nevertheless, closer and more orderly protein aggregates with denser network were founded in pidan white treated with 5.5 % KOH.
在长达4周的腌制过程中,监测了用4.5%氢氧化钠或5.5%氢氧化钾处理的皮蛋蛋白的化学成分、物理性质和微观结构变化,随后再陈化2周。随着腌制和陈化时间的增加,两种处理方式(4.5%氢氧化钠和5.5%氢氧化钾)下皮蛋蛋白的水分含量均降低,盐分含量增加(P<0.05)。用4.5%氢氧化钠处理的皮蛋蛋白的游离碱度和pH值在腌制过程中升高,但在两种腌制处理的陈化过程中均降低(P<0.05)。在腌制第4周时,用5.5%氢氧化钾处理的皮蛋蛋白比用4.5%氢氧化钠处理的皮蛋蛋白具有更高的硬度、凝聚性、胶黏性和咀嚼性。陈化后,用5.5%氢氧化钾处理的皮蛋蛋白通常具有更高的弹性、弹性模量(G')和黏性模量(G")(P<0.05)。随着腌制时间的增加,两种处理方式下的皮蛋蛋白的L*、b值降低,a值升高(P<0.05)。通过扫描电子显微镜观察可知,无论采用何种腌制处理方式,皮蛋蛋白凝胶中均发生了卵蛋白的聚集。然而,在用5.5%氢氧化钾处理的皮蛋蛋白中发现了更紧密、更有序且网络更致密的蛋白聚集体。