雌二醇对雄性和雌性大鼠肾缺血再灌注所致肾损伤的预防作用
Preventive role of estradiol on kidney injury induced by renal ischemia-reperfusion in male and female rats.
作者信息
Iran-Nejad Akram, Nematbakhsh Mehdi, Eshraghi-Jazi Fatemeh, Talebi Ardeshir
机构信息
Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran ; Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran ; Isfahan Institute of Basic and Applied Sciences Research, Isfahan, Iran.
出版信息
Int J Prev Med. 2015 Mar 20;6:22. doi: 10.4103/2008-7802.153537. eCollection 2015.
BACKGROUND
Renal ischemia-reperfusion (RIR) is the main cause of renal failure. The incidence of RIR injury seems to be gender-related due to female sex hormone; estrogen. This study was designed to investigate the protective role of estrogen against RIR injury in male and ovariectomized female rats.
METHODS
Thirty-nine Wistar rats were used in this study as male and ovariectomized female rats in the sham-operated, RIR, and estradiol-treated plus RIR groups. The RIR was induced by clamping the renal vessels for 45 min and then 24 h of reperfusion. All animals finally were sacrificed for the measurements.
RESULTS
The serum levels of creatinine and blood urea nitrogen and kidney tissue damage score significantly increased in both male and female RIR rats (P < 0.05). Estradiol however significantly attenuated theses parameters (P < 0.05) toward normal levels in female (P < 0.05), but not in male rats. Kidney weight increased in both genders and estradiol intensified it in the male rats (P < 0.05). Uterus weight was increased by estradiol in female rats (P < 0.05) and testis weight did not alter in male rats.
CONCLUSIONS
Estradiol demonstrated a protective role against RIR injury in female rats; however, estradiol as an antioxidant could not protect the male kidney from RIR injury.
背景
肾缺血再灌注（RIR）是肾衰竭的主要原因。由于女性性激素——雌激素，RIR损伤的发生率似乎与性别有关。本研究旨在探讨雌激素对雄性和去卵巢雌性大鼠RIR损伤的保护作用。
方法
本研究使用39只Wistar大鼠，分为假手术组、RIR组和雌二醇治疗加RIR组的雄性和去卵巢雌性大鼠。通过夹闭肾血管45分钟，然后再灌注24小时诱导RIR。所有动物最终均处死后进行测量。
结果
雄性和雌性RIR大鼠的血清肌酐和血尿素氮水平以及肾组织损伤评分均显著升高（P<0.05）。然而，雌二醇使雌性大鼠的这些参数显著降低至正常水平（P<0.05），但对雄性大鼠无此作用。两性的肾脏重量均增加，雌二醇使雄性大鼠的肾脏重量进一步增加（P<0.05）。雌二醇使雌性大鼠的子宫重量增加（P<0.05），而雄性大鼠的睾丸重量未改变。
结论
雌二醇对雌性大鼠的RIR损伤具有保护作用；然而，雌二醇作为抗氧化剂不能保护雄性肾脏免受RIR损伤。
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