Moeini Maryam, Nematbakhsh Mehdi, Fazilati Mohammad, Talebi Ardeshir, Pilehvarian Ali Asghar, Azarkish Fariba, Eshraghi-Jazi Fatemeh, Pezeshki Zahra
Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran ; Department of Biochemistry, Isfahan University of Payame-Noor, Isfahan, Iran.
Int J Prev Med. 2013 Jun;4(6):648-55.
Acute kidney injury (AKI) has been recognized as one of the most complex clinical complications in modern medicine, and ischemia/reperfusion (I/R) injury is well-known as a main reason of AKI. In addition, AKI leads to important systemic consequences such as acute lung injury. This study was designed to investigate the role of erythropoietin (EPO) on kidney function makers and tissue damage; and lung endothelial permeability and lung water content (LWC) in bilateral renal I/R injury model in rats.
Male Wistar rats were randomly divided into three groups of sham, I/R, and I/R treated with EPO (I/R + EPO) groups. The I/R and I/R + EPO groups were subjected to bilateral renal I/R injury; however, only the I/R + EPO group received EPO (500 IU/kg, i.p.) 2 h before ischemia surgery, and the same dose was continued once a day for 3 days after ischemia. The sham group underwent a surgical procedure without ischemia process.
The blood urea nitrogen (BUN) and serum creatinine (Cr) levels, kidney tissue damage score (KTDS), and kidney weight (KW) per 100 g body weight significantly increased in I/R group (P < 0.05). EPO administration decreased levels of BUN and Cr significantly (P < 0.05), and KTDS and KW insignificantly (P = 0.1). No significant differences in kidney and serum levels of malondialdehyde, and lung vascular permeability and LWC were observed between the groups. The serum and kidney levels of nitrite were not significantly different between I/R and sham groups; however, administration of EPO increased the renal level of nitrite (P < 0.05).
EPO protected the kidney against I/R injury; however, it may not protect the lung tissue from the damage induced by renal I/R injury in rats.
急性肾损伤（AKI）已被公认为现代医学中最复杂的临床并发症之一，而缺血/再灌注（I/R）损伤是AKI的一个主要原因。此外，AKI会导致诸如急性肺损伤等重要的全身后果。本研究旨在探讨促红细胞生成素（EPO）在大鼠双侧肾I/R损伤模型中对肾功能指标和组织损伤、肺内皮通透性及肺含水量（LWC）的作用。
雄性Wistar大鼠随机分为假手术组、I/R组和EPO治疗的I/R组（I/R + EPO组）。I/R组和I/R + EPO组接受双侧肾I/R损伤；然而，只有I/R + EPO组在缺血手术前2小时腹腔注射EPO（500 IU/kg），缺血后每天继续给予相同剂量，共3天。假手术组进行无缺血过程的手术操作。
I/R组血尿素氮（BUN）和血清肌酐（Cr）水平、肾组织损伤评分（KTDS）以及每100克体重的肾重量（KW）显著升高（P < 0.05）。给予EPO后，BUN和Cr水平显著降低（P < 0.05），KTDS和KW无显著变化（P = 0.1）。各组之间丙二醛的肾和血清水平、肺血管通透性及LWC无显著差异。I/R组和假手术组之间血清和肾中亚硝酸盐水平无显著差异；然而，给予EPO可提高肾中亚硝酸盐水平（P < 0.05）。
EPO可保护肾脏免受I/R损伤；然而，它可能无法保护大鼠肺组织免受肾I/R损伤诱导的损伤。
