Mazaheri Bahar, Emami Fatemeh, Moslemi Fatemeh, Talebi Ardeshir, Nematbakhsh Mehdi
Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Clinical Pathology, Isfahan University of Medical Sciences, Isfahan, Iran.
Malays J Med Sci. 2019 Jul;26(4):39-46. doi: 10.21315/mjms2019.26.4.5. Epub 2019 Aug 29.
Renal ischemia/reperfusion (RIR) is a major cause of kidney dysfunction in clinic. The main objective of this study was to investigate the effect of pre-conditioning ischemia (IPC) and zinc (Zn) supplementation on renal RIR injury.
A total of 63 unilateral nephrectomised male and female Wistar rats were divided into five groups. Group 1 (ShOPR): Rats as sham-operated group were subjected to surgical procedure without RIR. Group 2 (Isch): Rats underwent RIR (left kidney ischemia for 30 min followed by 48 h reperfusion). Group 3 (Zn+Isch): Rats were treated as group 2 but they received Zn sulphate (30 mg/kg) 1 h before induction of RIR. Group 4 (IPC+Isch): Rats were treated as group 2 but they underwent 1 min of ischemia followed by 3 min reperfusion as IPC, which was repeated for three times before induction of RIR. Group 5 (Zn+IPC+Isch): Rats were subjected to receive both Zn sulphate and IPC before induction of RIR. Urine samples were collected in the last 6 h of reperfusion, and finally biochemical and histological measurements were performed.
The serum level of creatinine (Cr), normalised kidney weight (KW) and kidney tissue damage score (KTDS) increased by RIR alone significantly ( < 0.05). These parameters were attenuated statistically by Zn supplementation ( < 0.05). However, IPC alone or co-treatment of Zn and IPC did not improve the biochemical and histological markers altered by RIR injury.
Zn supplementation had a protective role against RIR while such protective effect was not observed by IPC alone or by co-treatment of Zn and IPC.
肾缺血/再灌注（RIR）是临床上导致肾功能障碍的主要原因。本研究的主要目的是探讨预处理缺血（IPC）和补充锌（Zn）对肾RIR损伤的影响。
总共63只接受单侧肾切除的雄性和雌性Wistar大鼠被分为五组。第1组（假手术组）：作为假手术组的大鼠接受了无RIR的手术操作。第2组（缺血组）：大鼠经历RIR（左肾缺血30分钟，随后再灌注48小时）。第3组（锌+缺血组）：大鼠的处理方式与第2组相同，但在诱导RIR前1小时接受硫酸锌（30毫克/千克）。第4组（IPC+缺血组）：大鼠的处理方式与第2组相同，但在诱导RIR前进行1分钟缺血，随后3分钟再灌注作为IPC，重复三次。第5组（锌+IPC+缺血组）：大鼠在诱导RIR前同时接受硫酸锌和IPC。在再灌注的最后6小时收集尿液样本，最后进行生化和组织学测量。
单独RIR显著增加了血清肌酐（Cr）水平、标准化肾重量（KW）和肾组织损伤评分（KTDS）（<0.05）。补充锌使这些参数在统计学上有所降低（<0.05）。然而，单独IPC或锌与IPC联合处理并未改善因RIR损伤而改变的生化和组织学指标。
补充锌对RIR具有保护作用，而单独IPC或锌与IPC联合处理未观察到这种保护作用。
