Henriksen M M M, Kania P W, Buchmann K, Dalsgaard I
J Fish Dis. 2015 Mar;38(3):259-70. doi: 10.1111/jfd.12232.
The immune response and morphological changes in the gills of rainbow trout fry after immersion in hydrogen peroxide (H2O2), Flavobacterium psychrophilum or combined exposure were examined. The gills were sampled 4, 48, 125 and 192 h after exposure, and the regulation of expression of the following genes was investigated using qPCR: IgT, IgM, CD8, CD4, MHC I, MHC II, IL-4/13A, TcR-β, IL-10, IL-1β, IL-17, SAA and FoxP3. Bacteria were not observed in haematoxylin-and-eosin-stained gill tissue, but the presence of F. psychrophilum 16S rRNA was detected using qPCR. The 16S rRNA levels were correlated with gene expression. Although pretreatment with H2O2 before immersion in F. psychrophilum did not significantly alter the amount of bacteria found in the gill, the immune response was influenced: exposure to F. psychrophilum resulted in a negative correlation with expression of IL-17c1, MHC I and MHC II, while pretreatment with H2O2 resulted in a positive correlation with IL-4/13A and IgM. Exposure to either H2O2 or F. psychrophilum influenced the regulation of gene expression and damaged tissue. Exposure to both combined altered the immune response to infection and postponed healing of gill tissue.
研究了虹鳟鱼苗在浸泡于过氧化氢（H₂O₂）、嗜冷黄杆菌或联合暴露后鳃部的免疫反应和形态变化。在暴露后4、48、125和192小时采集鳃样本，并使用qPCR研究以下基因表达的调控：IgT、IgM、CD8、CD4、MHC I、MHC II、IL-4/13A、TcR-β、IL-10、IL-1β、IL-17、SAA和FoxP3。在苏木精-伊红染色的鳃组织中未观察到细菌，但使用qPCR检测到嗜冷黄杆菌16S rRNA的存在。16S rRNA水平与基因表达相关。虽然在浸泡于嗜冷黄杆菌之前用H₂O₂预处理并未显著改变鳃中发现的细菌数量，但免疫反应受到影响：暴露于嗜冷黄杆菌导致与IL-17c1、MHC I和MHC II的表达呈负相关，而用H₂O₂预处理则导致与IL-4/13A和IgM呈正相关。暴露于H₂O₂或嗜冷黄杆菌都会影响基因表达的调控并损害组织。联合暴露于两者会改变对感染的免疫反应并延缓鳃组织的愈合。
