• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

干细胞条件培养基减轻了白蛋白诱导的肾小管细胞上皮-间质转化。

Stem cell conditioned culture media attenuated albumin-induced epithelial-mesenchymal transition in renal tubular cells.

作者信息

Hu Junping, Zhu Qing, Li Pin-Lan, Wang Weili, Yi Fan, Li Ningjun

机构信息

Department of Pharmacology & Toxicology, Virginia Commonwealth University School of Medicine, Richmond, VA, USA.

出版信息

Cell Physiol Biochem. 2015;35(5):1719-28. doi: 10.1159/000373984. Epub 2015 Mar 19.

DOI:10.1159/000373984
PMID:25832005
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4401473/
Abstract

BACKGROUND

Proteinuria-induced epithelial-mesenchymal transition (EMT) plays an important role in progressive renal tubulointerstitial fibrosis in chronic renal disease. Stem cell therapy has been used for different diseases. Stem cell conditioned culture media (SCM) exhibits similar beneficial effects as stem cell therapy. The present study tested the hypothesis that SCM inhibits albumin-induced EMT in cultured renal tubular cells.

METHODS

Rat renal tubular cells were treated with/without albumin (20 µmg/ml) plus SCM or control cell media (CCM). EMT markers and inflammatory factors were measured by Western blot and fluorescent images.

RESULTS

Albumin induced EMT as shown by significant decreases in levels of epithelial marker E-cadherin, increases in mesenchymal markers fibroblast-specific protein 1 and α-smooth muscle actin, and elevations in collagen I. SCM inhibited all these changes. Meanwhile, albumin induced NF-κB translocation from cytosol into nucleus and that SCM blocked the nuclear translocation of NF-κB. Albumin also increased the levels of pro-inflammatory factor monocyte chemoattractant protein-1 (MCP)-1 by nearly 30 fold compared with control. SCM almost abolished albumin-induced increase of MCP-1.

CONCLUSION

These results suggest that SCM attenuated albumin-induced EMT in renal tubular cells via inhibiting activation of inflammatory factors, which may serve as a new therapeutic approach for chronic kidney diseases.

摘要

背景

蛋白尿诱导的上皮-间质转化（EMT）在慢性肾脏病进行性肾小管间质纤维化中起重要作用。干细胞疗法已用于多种疾病。干细胞条件培养基（SCM）具有与干细胞疗法相似的有益作用。本研究检验了SCM抑制培养的肾小管细胞中白蛋白诱导的EMT这一假说。

方法

用/不用白蛋白（20µmg/ml）加SCM或对照细胞培养基（CCM）处理大鼠肾小管细胞。通过蛋白质印迹法和荧光图像测量EMT标志物和炎症因子。

结果

白蛋白诱导EMT，表现为上皮标志物E-钙黏蛋白水平显著降低、间质标志物成纤维细胞特异性蛋白1和α-平滑肌肌动蛋白增加以及I型胶原升高。SCM抑制了所有这些变化。同时，白蛋白诱导NF-κB从胞质溶胶转位至细胞核，而SCM阻断了NF-κB的核转位。与对照相比，白蛋白还使促炎因子单核细胞趋化蛋白-1（MCP）-1水平增加近30倍。SCM几乎消除了白蛋白诱导的MCP-1增加。

结论

这些结果表明，SCM通过抑制炎症因子激活减轻了白蛋白诱导的肾小管细胞EMT，这可能成为慢性肾脏病的一种新治疗方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8202/4401473/cb9f841aa1ae/nihms675730f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8202/4401473/1e73d2a99591/nihms675730f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8202/4401473/9a05b9f8757a/nihms675730f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8202/4401473/18dcf5106e81/nihms675730f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8202/4401473/ffc02c19e1d0/nihms675730f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8202/4401473/f3efe6c2130c/nihms675730f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8202/4401473/dbd25fcc01c1/nihms675730f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8202/4401473/cb9f841aa1ae/nihms675730f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8202/4401473/1e73d2a99591/nihms675730f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8202/4401473/9a05b9f8757a/nihms675730f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8202/4401473/18dcf5106e81/nihms675730f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8202/4401473/ffc02c19e1d0/nihms675730f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8202/4401473/f3efe6c2130c/nihms675730f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8202/4401473/dbd25fcc01c1/nihms675730f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8202/4401473/cb9f841aa1ae/nihms675730f8.jpg

相似文献

1
Stem cell conditioned culture media attenuated albumin-induced epithelial-mesenchymal transition in renal tubular cells.干细胞条件培养基减轻了白蛋白诱导的肾小管细胞上皮-间质转化。
Cell Physiol Biochem. 2015;35(5):1719-28. doi: 10.1159/000373984. Epub 2015 Mar 19.
2
Mesenchymal stem cells modulate albumin-induced renal tubular inflammation and fibrosis.间质干细胞调节白蛋白诱导的肾小管炎症和纤维化。
PLoS One. 2014 Mar 19;9(3):e90883. doi: 10.1371/journal.pone.0090883. eCollection 2014.
3
Arctigenin suppresses transforming growth factor-β1-induced expression of monocyte chemoattractant protein-1 and the subsequent epithelial-mesenchymal transition through reactive oxygen species-dependent ERK/NF-κB signaling pathway in renal tubular epithelial cells.牛蒡子苷元通过活性氧依赖性ERK/NF-κB信号通路抑制转化生长因子-β1诱导的肾小管上皮细胞中单核细胞趋化蛋白-1的表达及随后的上皮-间质转化。
Free Radic Res. 2015;49(9):1095-113. doi: 10.3109/10715762.2015.1038258. Epub 2015 May 13.
4
Albumin-induced epithelial mesenchymal transformation.白蛋白诱导的上皮间质转化。
Nephron Exp Nephrol. 2012;120(3):e91-102. doi: 10.1159/000336822. Epub 2012 May 16.
5
Hypoxia inducible factor-1α mediates the profibrotic effect of albumin in renal tubular cells.缺氧诱导因子-1α介导白蛋白在肾小管细胞中的促纤维化作用。
Sci Rep. 2017 Nov 20;7(1):15878. doi: 10.1038/s41598-017-15972-8.
6
Human umbilical cord mesenchymal stem cell conditioned medium attenuates renal fibrosis by reducing inflammation and epithelial-to-mesenchymal transition via the TLR4/NF-κB signaling pathway in vivo and in vitro.人脐带间充质干细胞条件培养基通过体内和体外 TLR4/NF-κB 信号通路减少炎症和上皮-间充质转化来减轻肾纤维化。
Stem Cell Res Ther. 2018 Jan 12;9(1):7. doi: 10.1186/s13287-017-0760-6.
7
Downregulation of microRNA-429 contributes to angiotensin II-induced profibrotic effect in rat kidney.下调 microRNA-429 有助于血管紧张素 II 诱导的大鼠肾脏成纤维细胞增殖。
Am J Physiol Renal Physiol. 2018 Dec 1;315(6):F1536-F1541. doi: 10.1152/ajprenal.00478.2017. Epub 2018 Aug 22.
8
The influence of fasudil on renal proximal tubular cell epithelial-mesenchymal transition induced by parathormone.法舒地尔对甲状旁腺激素诱导的肾近端小管上皮细胞上皮-间充质转化的影响。
Ren Fail. 2017 Nov;39(1):575-581. doi: 10.1080/0886022X.2017.1349677.
9
Pirfenidone suppresses MAPK signalling pathway to reverse epithelial-mesenchymal transition and renal fibrosis.吡非尼酮可抑制丝裂原活化蛋白激酶信号通路，以逆转上皮-间质转化和肾纤维化。
Nephrology (Carlton). 2017 Aug;22(8):589-597. doi: 10.1111/nep.12831.
10
Chrysin inhibits diabetic renal tubulointerstitial fibrosis through blocking epithelial to mesenchymal transition.白杨素通过阻断上皮-间质转化抑制糖尿病肾小管间质纤维化。
J Mol Med (Berl). 2015 Jul;93(7):759-72. doi: 10.1007/s00109-015-1301-3. Epub 2015 Jun 11.

引用本文的文献

1
Association between lactate-albumin ratio and 28-day mortality in patients with sepsis-associated acute kidney injury: a retrospective cohort study.脓毒症相关性急性肾损伤患者乳酸-白蛋白比值与28天死亡率的关系：一项回顾性队列研究
Sci Rep. 2025 Mar 24;15(1):10087. doi: 10.1038/s41598-025-94753-0.
2
Renal Medullary Overexpression of Sphingosine-1-Phosphate Receptor 1 Transgene Attenuates Deoxycorticosterone Acetate (DOCA)-Salt Hypertension.肾髓质鞘氨醇-1-磷酸受体 1 转基因过表达减轻脱氧皮质酮醋酸盐（DOCA）-盐高血压。
Am J Hypertens. 2023 Aug 5;36(9):509-516. doi: 10.1093/ajh/hpad046.
3
Role of SUMOylation of STAT1 in tubular epithelial‑mesenchymal transition induced by high glucose.

本文引用的文献

1
Prospect of stem cell conditioned medium in regenerative medicine.干细胞条件培养基在再生医学中的前景。
Biomed Res Int. 2014;2014:965849. doi: 10.1155/2014/965849. Epub 2014 Aug 28.
2
FTY720 inhibits tubulointerstitial inflammation in albumin overload-induced nephropathy of rats via the Sphk1 pathway.FTY720通过Sphk1途径抑制白蛋白过载诱导的大鼠肾病中的肾小管间质炎症。
Acta Pharmacol Sin. 2014 Dec;35(12):1537-45. doi: 10.1038/aps.2014.100. Epub 2014 Nov 17.
3
Inflammatory cytokines induce a reduction in E-cadherin expression and morphological changes in MDCK cells.
SUMOylation 修饰的 STAT1 在高糖诱导的肾小管上皮-间充质转分化中的作用。
Mol Med Rep. 2023 Feb;27(2). doi: 10.3892/mmr.2023.12929. Epub 2023 Jan 5.
4
Loss of sphingosine kinase 2 protects against cisplatin-induced kidney injury.鞘氨醇激酶 2 的缺失可预防顺铂诱导的肾损伤。
Am J Physiol Renal Physiol. 2022 Sep 1;323(3):F322-F334. doi: 10.1152/ajprenal.00229.2021. Epub 2022 Jul 14.
5
Apamin inhibits renal fibrosis via suppressing TGF-β1 and STAT3 signaling in vivo and in vitro.阿帕米胺通过体内和体外抑制 TGF-β1 和 STAT3 信号通路抑制肾纤维化。
J Mol Med (Berl). 2021 Sep;99(9):1265-1277. doi: 10.1007/s00109-021-02087-x. Epub 2021 May 24.
6
Characterization of the iPSC-derived conditioned medium that promotes the growth of bovine corneal endothelial cells.诱导多能干细胞来源的促进牛角膜内皮细胞生长的条件培养基的特性分析。
PeerJ. 2019 Apr 16;7:e6734. doi: 10.7717/peerj.6734. eCollection 2019.
7
Inhibition of p300/CBP-Associated Factor Attenuates Renal Tubulointerstitial Fibrosis through Modulation of NF-kB and Nrf2.抑制 p300/CBP 相关因子通过调节 NF-κB 和 Nrf2 减轻肾间质纤维化。
Int J Mol Sci. 2019 Mar 28;20(7):1554. doi: 10.3390/ijms20071554.
8
Establishment of renal proximal tubule cell lines derived from the kidney of p53 knockout mice.源自p53基因敲除小鼠肾脏的肾近端小管细胞系的建立。
Cytotechnology. 2019 Feb;71(1):45-56. doi: 10.1007/s10616-018-0261-1. Epub 2019 Jan 2.
9
FKBP51 promotes migration and invasion of papillary thyroid carcinoma through NF-κB-dependent epithelial-to-mesenchymal transition.FKBP51通过依赖NF-κB的上皮-间质转化促进甲状腺乳头状癌的迁移和侵袭。
Oncol Lett. 2018 Dec;16(6):7020-7028. doi: 10.3892/ol.2018.9517. Epub 2018 Sep 27.
10
Downregulation of microRNA-429 contributes to angiotensin II-induced profibrotic effect in rat kidney.下调 microRNA-429 有助于血管紧张素 II 诱导的大鼠肾脏成纤维细胞增殖。
Am J Physiol Renal Physiol. 2018 Dec 1;315(6):F1536-F1541. doi: 10.1152/ajprenal.00478.2017. Epub 2018 Aug 22.
炎性细胞因子可导致MDCK细胞中E-钙黏蛋白表达降低及形态改变。
Res Vet Sci. 2014 Apr;96(2):288-91. doi: 10.1016/j.rvsc.2014.02.005. Epub 2014 Feb 11.
4
Transforming growth factor-β and the progression of renal disease.转化生长因子-β与肾脏疾病的进展
Nephrol Dial Transplant. 2014 Feb;29 Suppl 1:i37-i45. doi: 10.1093/ndt/gft267. Epub 2013 Sep 12.
5
Paricalcitol attenuates 4-hydroxy-2-hexenal-induced inflammation and epithelial-mesenchymal transition in human renal proximal tubular epithelial cells.帕立骨化醇可减轻 4-羟基-2-己烯醛诱导的人肾小管上皮细胞炎症和上皮-间充质转化。
PLoS One. 2013 May 17;8(5):e63186. doi: 10.1371/journal.pone.0063186. Print 2013.
6
The relative contribution of paracine effect versus direct differentiation on adipose-derived stem cell transplantation mediated cardiac repair.旁分泌效应与直接分化对脂肪来源干细胞移植介导的心脏修复的相对贡献。
PLoS One. 2013;8(3):e59020. doi: 10.1371/journal.pone.0059020. Epub 2013 Mar 19.
7
Hypoxia-inducible factor prolyl-hydroxylase-2 mediates transforming growth factor beta 1-induced epithelial-mesenchymal transition in renal tubular cells.缺氧诱导因子脯氨酰羟化酶-2介导转化生长因子β1诱导的肾小管上皮细胞间质转化。
Biochim Biophys Acta. 2013 Jun;1833(6):1454-62. doi: 10.1016/j.bbamcr.2013.02.029. Epub 2013 Mar 1.
8
Ischemia-reperfusion injury: beneficial effects of mesenchymal stromal cells.缺血再灌注损伤：间充质基质细胞的有益作用。
Curr Opin Organ Transplant. 2013 Feb;18(1):34-43. doi: 10.1097/MOT.0b013e32835c2a05.
9
The inhibitory effect of chimeric decoy oligodeoxynucleotide against NF-κB and Sp1 in renal interstitial fibrosis.嵌合诱饵寡脱氧核苷酸对肾间质纤维化中 NF-κB 和 Sp1 的抑制作用。
J Mol Med (Berl). 2013 May;91(5):573-86. doi: 10.1007/s00109-012-0972-2. Epub 2012 Nov 1.
10
Stem cell conditioned medium improves acute lung injury in mice: in vivo evidence for stem cell paracrine action.干细胞条件培养基可改善小鼠急性肺损伤：干细胞旁分泌作用的体内证据。
Am J Physiol Lung Cell Mol Physiol. 2012 Dec 1;303(11):L967-77. doi: 10.1152/ajplung.00144.2011. Epub 2012 Sep 28.