Schoepfer R, Halvorsen S W, Conroy W G, Whiting P, Lindstrom J
Salk Institute for Biological Studies, San Diego, CA 92138.
FEBS Lett. 1989 Nov 6;257(2):393-9. doi: 10.1016/0014-5793(89)81580-7.
Neuronal nicotinic acetylcholine receptor (AChR) subtypes have been defined pharmacologically, immunologically, and by DNA cloning, but the correlations between these approaches are incomplete. Vertebrate neuronal AChRs that have been isolated are composed of structural subunits and ACh-binding subunits. A single kind of subunit can be used in more than one AChR subtype. Monoclonal antibody (mAb) 35 binds to structural subunits of subtypes of AChRs from both chicken brain and ganglia. By using antisera to a unique sequence of alpha 3 ACh-binding subunits expressed in bacteria, we show that ganglionic AChRs contain alpha 3 ACh-binding subunits, whereas the brain AChR subtype that binds mAb 35 does not. Subunit-specific antisera raised against recombinant proteins should be a valuable approach for identifying the subunit composition of receptors in multigene, multisubunit families.
神经元烟碱型乙酰胆碱受体（AChR）亚型已通过药理学、免疫学及DNA克隆技术得以界定，但这些方法之间的关联并不完全。已分离出的脊椎动物神经元AChR由结构亚基和ACh结合亚基组成。单一类型的亚基可用于不止一种AChR亚型。单克隆抗体（mAb）35可与鸡脑和神经节中AChR亚型的结构亚基结合。通过使用针对细菌中表达的α3 ACh结合亚基独特序列的抗血清，我们发现神经节AChR含有α3 ACh结合亚基，而结合mAb 35的脑AChR亚型则不含。针对重组蛋白产生的亚基特异性抗血清应是鉴定多基因、多亚基家族中受体亚基组成的一种有价值的方法。
