反义寡核苷酸揭示神经元乙酰胆碱受体亚基的功能贡献
Functional contribution of neuronal AChR subunits revealed by antisense oligonucleotides.
作者信息
Listerud M, Brussaard A B, Devay P, Colman D R, Role L W
机构信息
Department of Anatomy and Cell Biology, Columbia College of Physicians and Surgeons, New York, NY 10032.
出版信息
Science. 1991 Dec 6;254(5037):1518-21. doi: 10.1126/science.1720573.
Abstract
Although multiple related genes encoding nicotinic acetylcholine receptor (AChR) subunits have been identified, how each of these subunits contributes to AChRs in neurons is not known. Sympathetic neurons express four classes of AChR channels and six AChR subunit genes (alpha 3, alpha 4, alpha 5, alpha 7, beta 2, and beta 4). The contribution of individual subunits to AChR channel subtypes in these neurons was examined by selective deletion with antisense oligonucleotides. An alpha 3 antisense oligonucleotide decreased the number and altered the properties of the normally expressed ACh-activated channels. The remaining AChR channels have distinct biophysical and pharmacological properties that indicate an important functional contribution of the alpha 7 subunit.
摘要
尽管已经鉴定出多个编码烟碱型乙酰胆碱受体（AChR）亚基的相关基因，但这些亚基中的每一个如何在神经元中对AChR起作用尚不清楚。交感神经元表达四类AChR通道和六个AChR亚基基因（α3、α4、α5、α7、β2和β4）。通过用反义寡核苷酸进行选择性缺失，研究了单个亚基对这些神经元中AChR通道亚型的贡献。α3反义寡核苷酸减少了正常表达的ACh激活通道的数量并改变了其特性。其余的AChR通道具有独特的生物物理和药理学特性，这表明α7亚基具有重要的功能作用。
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