De Maglio Giovanna, Casagrande Mariaelena, Guardascione Michela, Fontanella Caterina, Lutrino Stefania Eufemia, Rihawi Karim, Pisa Federica Edith, Tuniz Francesco, Fasola Gianpiero, Pizzolitto Stefano, Aprile Giuseppe
Department of Pathology, University and General Hospital, Udine, Italy.
Future Oncol. 2015;11(8):1201-9. doi: 10.2217/fon.15.2.
Brain metastases (BM) from colorectal cancer are usually associated with poor prognosis. The aim of this retrospective study is to evaluate MGMT promoter methylation in BM and their corresponding primary colorectal cancer tumors.
MATERIALS & METHODS: MGMT promoter methylation status was assessed by pyrosequencing in 53 consecutive patients resected for BM. A concordance analysis between BM and matched primary tumor was performed in 39 cases.
MGMT methylation was found in 34 (64.2%) BM and in 25 corresponding primary tumors (64.1%). Median survival after neurosurgery was independent from MGMT promoter methylation (163 days for those with methylated MGMT versus 193 days for the unmethylated).
Epigenetic MGMT promoter methylation was common and the concordance between primary and secondary lesions was high.
结直肠癌脑转移（BM）通常与预后不良相关。本回顾性研究的目的是评估BM及其相应的原发性结直肠癌肿瘤中MGMT启动子甲基化情况。
通过焦磷酸测序法评估了53例因BM接受手术切除的连续患者的MGMT启动子甲基化状态。对39例患者的BM与配对的原发性肿瘤进行了一致性分析。
在34例（64.2%）BM及25例相应的原发性肿瘤（64.1%）中发现了MGMT甲基化。神经外科手术后的中位生存期与MGMT启动子甲基化无关（MGMT甲基化者为163天，未甲基化者为193天）。
表观遗传学上的MGMT启动子甲基化很常见，原发性和继发性病变之间的一致性很高。
