Restuccia Donatella, Spizzirri U Gianfranco, Puoci Francesco, Picci Nevio
a Department of Pharmacy, Health and Nutritional Sciences , University of Calabria , Arcavacata di Rende (CS) , Italy.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2015;32(7):1156-63. doi: 10.1080/19440049.2015.1036322. Epub 2015 Apr 24.
Cocoa contains many compounds such as biogenic amines (BAs), known to influence consumer health. Spermidine, spermidine, putrescine, histamine, tyramine, β-phenylethylamine, cadaverine and serotonine have been found in several cocoa-based products using HPLC with UV detection after derivatisation with dansyl-chloride. Once optimised in terms of linearity, percentage recovery, LOD, LOQ and repeatability, this method was applied to real samples. Total concentrations of BAs ranged from 5.7 to 79.0 µg g(-)(1) with wide variations depending on the type of sample. BAs present in all samples were in decreasing order: histamine (1.9-38.1 µg g(-)(1)) and tyramine (1.7-31.7 µg g(-)(1)), while putrescine (0.9-32.7 µg g(-)(1)), spermidine (1.0-9.7 µg g(-)(1)) and spermidine (0.6-9.3 µg g(-)(1)) were present in most of the samples. Cadaverine, serotonine and β-phenylethylamine were present in a few samples at much lower concentrations. Organic samples always contained much lower levels of BAs than their conventional counterparts and, generally speaking, the highest amounts of BAs were found in the most processed products.
可可含有许多化合物，如生物胺（BAs），已知这些化合物会影响消费者健康。使用丹磺酰氯衍生化后通过带紫外检测的高效液相色谱法（HPLC），已在几种可可基产品中发现了亚精胺、精胺、腐胺、组胺、酪胺、β-苯乙胺、尸胺和血清素。该方法在线性、回收率百分比、检测限、定量限和重复性方面优化后，应用于实际样品。生物胺的总浓度范围为5.7至79.0 μg g⁻¹，因样品类型而异，差异很大。所有样品中存在的生物胺按含量递减顺序为：组胺（1.9 - 38.1 μg g⁻¹）和酪胺（1.7 - 31.7 μg g⁻¹），而腐胺（0.9 - 32.7 μg g⁻¹）、亚精胺（1.0 - 9.7 μg g⁻¹）和精胺（0.6 - 9.3 μg g⁻¹）存在于大多数样品中。尸胺、血清素和β-苯乙胺在少数样品中以低得多的浓度存在。有机样品中的生物胺含量总是远低于其传统对应物，一般来说，在加工程度最高的产品中发现的生物胺含量最高。
