人线粒体NADH脱氢酶24kDa铁硫蛋白前体编码cDNA的克隆与测序

Cloning and sequencing of a cDNA encoding the precursor to the 24 kDa iron-sulfur protein of human mitochondrial NADH dehydrogenase.

Toda H, Hosokawa Y, Nishikimi M, Suzuki H, Kato K, Ozawa T

Department of Biomedical Chemistry, Faculty of Medicine, University of Nagoya, Japan.

Int J Biochem. 1989;21(10):1161-8. doi: 10.1016/0020-711x(89)90060-8.

Abstract

We have isolated a cDNA encoding the 24 kDa subunit, an iron-sulfur protein, of mitochondrial NADH dehydrogenase from a human fibroblast cDNA library by colony hybridization using a rat 24 kDa subunit cDNA as a probe. 2. The presequence predicted from the human cDNA sequence is typical of precursors to mitochondrial proteins in a high content of basic residues and in the absence of acidic ones. 3. The mature form of the human 24 kDa subunit shows 95% homology with its rat counterpart. Five cysteine residues are conserved among human, rat and bovine; four of these are expected to be involved in the binding of a binuclear iron-sulfur cluster.

摘要

我们使用大鼠24 kDa亚基cDNA作为探针，通过菌落杂交从人成纤维细胞cDNA文库中分离出编码线粒体NADH脱氢酶24 kDa亚基（一种铁硫蛋白）的cDNA。2. 从人cDNA序列预测的前导序列是线粒体蛋白前体的典型序列，碱性残基含量高且无酸性残基。3. 人24 kDa亚基的成熟形式与其大鼠对应物具有95%的同源性。人、大鼠和牛中五个半胱氨酸残基保守；其中四个预计参与双核铁硫簇的结合。