Usui S, Yu L, Yu C A
Department of Biochemistry, OAES, Oklahoma State University, Stillwater 74078.
Biochem Biophys Res Commun. 1990 Mar 16;167(2):575-9. doi: 10.1016/0006-291x(90)92063-6.
Two cDNA clones encoding bovine heart mitochondrial Rieske iron-sulfur protein were obtained by immunological screening of a bovine heart cDNA expression library in lambda gt11 with antiserum directed against Rieske iron-sulfur protein isolated from bovine heart mitochondrial ubiquinol-cytochrome c reductase. The cDNA inserts were 1005 and 1100 base pairs with an open reading frame of 807 base pairs which encoded a 196-amino acid mature Rieske iron-sulfur protein and a 73-amino acid presequence. The amino acid sequence of Rieske iron-sulfur protein deduced from nucleotide sequencing is the same as that obtained from protein sequencing except at residues #73 and #191 which are Ser and Asp instead of Ala and Gly, respectively.
通过用针对从牛心线粒体泛醇 - 细胞色素c还原酶中分离出的 Rieske 铁硫蛋白的抗血清对λgt11 中的牛心 cDNA 表达文库进行免疫筛选，获得了两个编码牛心线粒体 Rieske 铁硫蛋白的 cDNA 克隆。cDNA 插入片段分别为 1005 和 1100 个碱基对，有一个 807 个碱基对的开放阅读框，编码一个 196 个氨基酸的成熟 Rieske 铁硫蛋白和一个 73 个氨基酸的前导序列。从核苷酸测序推导的 Rieske 铁硫蛋白的氨基酸序列与从蛋白质测序获得的序列相同，只是在第 73 和 191 位残基处不同，分别为 Ser 和 Asp 而非 Ala 和 Gly。
