柯克曼-罗宾斯肝癌中非组蛋白染色质蛋白的生长相关变化。

Growth-related changes of non-histone chromatin proteins from Kirkman-Robbins hepatoma.

Kilianska Z, Krajewska W M, Gaczynski M, Marszalek M, Klyszejko-Stefanowicz L

Department of Cytobiochemistry, University of Lódź, Poland.

Int J Biochem. 1989;21(8):873-81. doi: 10.1016/0020-711x(89)90286-3.

Abstract

Non-histone chromatin protein fractions NHCP1 and NHCP2 eluted from hydroxyapatite with 50 and 100 mM phosphate buffer (pH 6.8) from nuclei of Kirkman-Robbins hepatoma from 4th, 7th and 9th day of growth were analysed by one- and two-dimensional gel electrophoresis as well as Western blot technique in the presence of antibodies elicited against NHCP1, NHCP2 and dehistonized chromatin of hamster hepatoma and liver. 2. The presence of electrophoretically and immunologically specific components among NHCP1 and NHCP2 fractions during Kirkman-Robbins hepatoma growth was stated.

摘要

从生长第4天、第7天和第9天的柯克曼 - 罗宾斯肝癌细胞核中，用50 mM和100 mM磷酸盐缓冲液（pH 6.8）从羟基磷灰石上洗脱的非组蛋白染色质蛋白组分NHCP1和NHCP2，在针对NHCP1、NHCP2以及仓鼠肝癌和肝脏的去组蛋白染色质产生的抗体存在的情况下，通过一维和二维凝胶电泳以及蛋白质免疫印迹技术进行分析。2. 指出在柯克曼 - 罗宾斯肝癌生长过程中，NHCP1和NHCP2组分中存在电泳和免疫特异性成分。