Trucco G, Fritsch R, Giorda R, Trucco M
Department of Pathology, University of Pittsburgh, School of Medicine, Pennsylvania.
Diabetes. 1989 Dec;38(12):1617-22. doi: 10.2337/diab.38.12.1617.
The allelic forms of the human leukocyte antigen (HLA)-DQ beta-chain (DQB1) have been recognized as the best markers of insulin-dependent diabetes mellitus (IDDM) susceptibility. We describe a method that allows the recognition of these DQB1 alleles without the use of either allele-specific oligonucleotide probes or radioactive material. This method determines these alleles by electrophoretically separating restriction enzyme-generated fragments from the polymerase chain-reaction-amplified second exon of the HLA-DQB1 gene, which encodes the first domain of the protein chain. This digestion method, which is simpler and more rapid than the previously adopted hybridization method, is described in detail to enable individuals at any clinical laboratory to quickly ascertain IDDM susceptibility.
人类白细胞抗原（HLA）-DQβ链（DQB1）的等位基因形式已被公认为胰岛素依赖型糖尿病（IDDM）易感性的最佳标志物。我们描述了一种方法，该方法无需使用等位基因特异性寡核苷酸探针或放射性物质就能识别这些DQB1等位基因。此方法通过对聚合酶链反应扩增的HLA-DQB1基因第二外显子的限制性内切酶产生的片段进行电泳分离来确定这些等位基因，该外显子编码蛋白质链的第一个结构域。这种消化方法比先前采用的杂交方法更简单、更快速，现详细介绍，以使任何临床实验室的人员都能快速确定IDDM易感性。
