Zeringer Emily, Barta Timothy, Li Mu, Vlassov Alexander V
Thermo Fisher Scientific, Austin, Texas 78744.
Cold Spring Harb Protoc. 2015 Apr 1;2015(4):319-23. doi: 10.1101/pdb.top074476.
Exosomes are tiny vesicles (diameter 30-150 nm) secreted by cells in culture and found in all body fluids. These vesicles, loaded with unique RNA and protein cargos, have many biological functions, of which only a small fraction is currently understood-for example, they participate in cell-to-cell communication and signaling within the human body. The spectrum of current scientific interest in exosomes is wide and ranges from understanding their functions and pathways to using them in diagnostics, as biomarkers, and in the development of therapeutics. Here we provide an overview of different strategies for isolation of exosomes from cell-culture media and body fluids.
外泌体是细胞在培养过程中分泌的微小囊泡（直径30 - 150纳米），存在于所有体液中。这些囊泡装载着独特的RNA和蛋白质货物，具有多种生物学功能，目前人们仅了解其中一小部分——例如，它们参与人体细胞间的通讯和信号传导。当前科学界对外泌体的兴趣范围广泛，从了解其功能和途径到将其用于诊断、作为生物标志物以及用于治疗药物开发。在此，我们概述了从细胞培养基和体液中分离外泌体的不同策略。
