Zhang Xia, Chen Yingping, Zhang Ting, Zhang Yaming
Yancheng City No.1 People's Hospital, Yancheng, Jiangsu Province, People's Republic of China.
Afr Health Sci. 2015 Mar;15(1):97-100. doi: 10.4314/ahs.v15i1.13.
Da Huang (Radix et Rhizoma Rhei) is the dried root or rhizome of Rheum palmatum L., Rheum tanguticum Maxim ex Balf. or Rheum officinale Braill of family Polygonaceae. It has heat clearing, damp drying, fire purging and toxin removing effects. Because of its definite curative efficacy, it has been widely applied in clinical settings.
To study the inhibitory effect of emodin on human hepatoma cell line SMMC-7721 and its mechanism.
MTT assay, flow cytometry and electron microscopy were used to investigate the inhibitory effect of different concentrations of emodin on human hepatoma cell line SMMC-7721.
12 h, 24 h and 48 h after the action of 20, 40 and 80 umol/L emodin on SMMC-7721 cells, the proliferation of human hepatoma SMMC-7721 cells was inhibited; the inhibitory effects showed time-and concentration-dependence. 48 h after the action of different concentrations of emodin on SMMC-7721 cells, cells in G2/M phase increased significantly, while the proportion of S phase cells gradually declined.
Emodin can inhibit human hepatoma cell line SMMC-7721.
大黄为蓼科植物掌叶大黄、唐古特大黄或药用大黄的干燥根及根茎。具有清热泻火、燥湿解毒之功效。因其确切的疗效，已在临床中广泛应用。
研究大黄素对人肝癌细胞系SMMC - 7721的抑制作用及其机制。
采用MTT法、流式细胞术和电子显微镜观察不同浓度大黄素对人肝癌细胞系SMMC - 7721的抑制作用。
20、40和80 μmol/L大黄素作用于SMMC - 7721细胞12 h、24 h和48 h后，人肝癌SMMC - 7721细胞增殖受到抑制；抑制作用呈时间和浓度依赖性。不同浓度大黄素作用于SMMC - 7721细胞48 h后，G2/M期细胞显著增多，而S期细胞比例逐渐下降。
大黄素可抑制人肝癌细胞系SMMC - 7721。
