Filichkin Sergei, Priest Henry D, Megraw Molly, Mockler Todd C
Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, USA; Center for Genome Research and Biocomputing, Oregon State University, Corvallis, OR 97331, USA.
Division of Biology and Biomedical Sciences, Washington University, Saint Louis, MO 63130, USA; Donald Danforth Plant Science Center, Saint Louis, MO 63132, USA.
Curr Opin Plant Biol. 2015 Apr;24:125-35. doi: 10.1016/j.pbi.2015.02.008. Epub 2015 Mar 30.
In recent years, high-throughput sequencing-based analysis of plant transcriptomes has suggested that up to ∼60% of plant gene loci encode alternatively spliced mature transcripts. These studies have also revealed that alternative splicing in plants can be regulated by cell type, developmental stage, the environment, and the circadian clock. Alternative splicing is coupled to RNA surveillance and processing mechanisms, including nonsense mediated decay. Recently, non-protein-coding transcripts have also been shown to undergo alternative splicing. These discoveries collectively describe a robust system of post-transcriptional regulatory feedback loops which influence RNA abundance. In this review, we summarize recent studies describing the specific roles alternative splicing and RNA surveillance play in plant adaptation to environmental stresses and the regulation of the circadian clock.
近年来，基于高通量测序的植物转录组分析表明，高达约60%的植物基因座编码可变剪接的成熟转录本。这些研究还揭示，植物中的可变剪接可受细胞类型、发育阶段、环境和生物钟调控。可变剪接与RNA监测及加工机制相关联，包括无义介导的衰变。最近，非蛋白质编码转录本也被证明会发生可变剪接。这些发现共同描述了一个强大的转录后调控反馈环系统，该系统影响RNA丰度。在本综述中，我们总结了近期的研究，这些研究描述了可变剪接和RNA监测在植物适应环境胁迫及生物钟调控中所起的具体作用。
