猿猴腺病毒30型反向末端重复序列的特征分析

Characterization of the simian adenovirus type 30 inverted terminal repeat.

作者信息

Cheng S M, Blume M, Vogel R, Hung C, Lee S G, Hung P P

机构信息

Biotechnology and Microbiology Division, Wyeth-Ayerst Research, Radnor, PA 19087.

出版信息

Gene. 1989 Aug 15;80(2):381-4. doi: 10.1016/0378-1119(89)90303-x.

DOI:10.1016/0378-1119(89)90303-x

PMID:2583517

Abstract

The presence of an inverted terminal repeat (ITR), which plays an important role in the initiation of DNA replication, is one of the characteristic properties of adenoviruses (Ads). We have established the nucleotide (nt) sequences for the ITR of simian adenovirus type 30 (SV30), a subgroup-III oncogenic virus. This repeat consists of 185 nt, representing the longest ITR found in an Ad so far. It contains multiple copies of internal repeats, as well as the consensus sequences of the putative binding sites for replication and transcription factors. The conserved features of the known ITRs are also found in SV30. Interestingly, the ITR of SV30 is more closely related to that of Ad5 (human), than to that of SA7 (simian).

摘要

反向末端重复序列（ITR）的存在是腺病毒（Ads）的特征性属性之一，它在DNA复制起始过程中发挥重要作用。我们已经确定了猿猴腺病毒30型（SV30，一种III亚组致癌病毒）ITR的核苷酸（nt）序列。这个重复序列由185个核苷酸组成，是迄今为止在腺病毒中发现的最长的ITR。它包含多个内部重复序列拷贝，以及复制和转录因子假定结合位点的共有序列。已知ITR的保守特征在SV30中也有发现。有趣的是，SV30的ITR与Ad5（人类）的ITR比与SA7（猿猴）的ITR关系更密切。