Feldstein P A, Buzayan J M, Bruening G
Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis 95616.
Gene. 1989 Oct 15;82(1):53-61. doi: 10.1016/0378-1119(89)90029-2.
Circular and multimeric forms of the satellite RNA of tobacco ringspot virus and their autolytic processing reactions are well known. They suggest replication models in which key elements are rolling circle transcription and the processing of the resulting multimeric RNA to generate the unit, 'monomeric' satellite RNA sequence. We prepared plasmids bearing two distinct sequences of the satellite RNA. Each was arranged to allow transcription of an oligoribonucleotide (r-oligo) of the polarity that is complementary to encapsidated satellite RNA. One sequence has the autolytic processing phosphodiester bond, ApG, and the other is located at a distance of about 150 nucleotide (nt) residues. The second r-oligo accomplished cleavage of the first, in a catalytic fashion. Analysis of truncated forms showed that 10 nt of the ApG junction-containing r-oligo and 46 of the endoribonucleolytic r-oligo were sufficient for recognition in the cleavage reaction. These results map the sequences involved in autolytic processing of the complementary polarity satellite RNA to two regions.
烟草环斑病毒卫星RNA的环状和多聚体形式及其自溶加工反应是众所周知的。它们提示了复制模型，其中关键要素是滚环转录以及对产生的多聚体RNA进行加工以生成单位“单体”卫星RNA序列。我们制备了携带卫星RNA两个不同序列的质粒。每个质粒都经过安排，以允许转录与衣壳化卫星RNA极性互补的寡核糖核苷酸（r-寡核苷酸）。一个序列具有自溶加工磷酸二酯键ApG，另一个位于约150个核苷酸（nt）残基的距离处。第二个r-寡核苷酸以催化方式完成了第一个的切割。对截短形式的分析表明，含ApG连接的r-寡核苷酸的10个nt和核糖核酸内切酶r-寡核苷酸的46个nt足以在切割反应中进行识别。这些结果将互补极性卫星RNA自溶加工所涉及的序列定位到两个区域。
