Zal Fatemeh, Mahdian Zeinab, Zare Razieh, Soghra Bahmanpour, Mostafavi-Pour Zohreh
Reproductive Biology Department, School of advanced medical sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran, and Infertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Recombinant Protein Lab in Biochemistry Department, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
Int J Vitam Nutr Res. 2014;84(1-2):55-64. doi: 10.1024/0300-9831/a000193.
This study was designed to assess oxidative damage and cell apoptosis in the uterus of rats with streptozotocin (STZ)-induced diabetes. The role of vitamin E (VE) and/or folic acid (FA) in the protection from such damage was also evaluated. The treatments were performed for 4 weeks on six groups of rats: 1) normal control 2) diabetic control 3) diabetic rats receiving olive oil as a vehicle (without VE) 4) diabetic rats treated with VE (200 mg/kg) in olive oil 5) diabetic rats treated with FA (25 mg/kg) and 6) diabetic rats treated with VE+FA (200 and 25 mg/kg, respectively). We measured the malondialdehyde level (MDA), glutathione content (GSH) and the activity of GSH peroxidase (GPx), GSH reductase (GR) and catalase. Changes in caspase-3 activity were quantified in uterine tissue to assess the rate of apoptosis. In the rat uterine tissues, MDA content and caspase-3 activity were significantly elevated, while GPx, GR and CAT activities and the GSH level were significantly decreased in the diabetic control compared with those in normal rats (p<0.05). The combination of the vitamins (VE+FA) restored uterine GSH content and enzymatic activities of GPx, GR and CAT and reduced the MDA level (p<0.05). A prominent reduction in apoptosis of uterine cells was detected in diabetic rats treated with two vitamins (p<0.05). Overall, VE alone, not FA, produced results similar to those of the VE+FA combination. Thus, in the uterine tissue of diabetic rats, diabetes complications (that are caused by oxidative damage and apoptosis induction) can be prevented by the systemic administration of VE and FA.
本研究旨在评估链脲佐菌素（STZ）诱导的糖尿病大鼠子宫中的氧化损伤和细胞凋亡情况。同时还评估了维生素E（VE）和/或叶酸（FA）在预防此类损伤中的作用。对六组大鼠进行了为期4周的治疗：1）正常对照组；2）糖尿病对照组；3）接受橄榄油作为载体（不含VE）的糖尿病大鼠；4）用橄榄油中VE（200mg/kg）治疗的糖尿病大鼠；5）用FA（25mg/kg）治疗的糖尿病大鼠；6）用VE+FA（分别为200和25mg/kg）治疗的糖尿病大鼠。我们测量了丙二醛水平（MDA）、谷胱甘肽含量（GSH）以及谷胱甘肽过氧化物酶（GPx）、谷胱甘肽还原酶（GR）和过氧化氢酶的活性。对子宫组织中caspase-3活性的变化进行定量，以评估凋亡率。在大鼠子宫组织中，与正常大鼠相比，糖尿病对照组的MDA含量和caspase-3活性显著升高，而GPx、GR和CAT活性以及GSH水平显著降低（p<0.05）。维生素组合（VE+FA）恢复了子宫GSH含量以及GPx、GR和CAT的酶活性，并降低了MDA水平（p<0.05）。在用两种维生素治疗的糖尿病大鼠中，检测到子宫细胞凋亡明显减少（p<0.05）。总体而言，单独使用VE而非FA产生的结果与VE+FA组合相似。因此，在糖尿病大鼠的子宫组织中，全身给予VE和FA可预防由氧化损伤和诱导凋亡引起的糖尿病并发症。
