Kinalski M, Telejko B, Zarzycki W, Górski J, Kinalska I
Zakładu Patofizjologii Ciazy Instytutu Połoznictwa i Chorób Kobiecych Akademii Medycznej w Białymstoku.
Przegl Lek. 1998;55(6):320-4.
The aim of our study was to establish if moderate doses of nonenzymatic antioxidant, vitamin E, could play a role in the antioxidant defence system in pregnant diabetic rats and their offspring. Two models of experimental diabetes were used: the 1st group consisted of 30 female Wistar rats which were given a single dose of streptozotocin (40 mg/kg) and were mated 7 days later, whereas in the 2nd group diabetes was induced on the 7th day of pregnancy (using the same dose of streptozotocin). In each part of the experiment diabetic animals were divided into two equal groups: vitamin E supplemented and fed with standard diet only. Our controls consisted of 20 normal rats. On the 1 day after delivery rats were decapitated and homogenates of maternal liver and uterus as well as neonatal lungs and liver were prepared. Then the following parameters were measured: malondialdehyde (MDA) concentrations in the homogenates and blood serum (colorimetric assay), glutathione (GSH) levels (colorimetric test), the activity of CuZn superoxide dismutase (SOD) (spectrophotometric method) and glutathione peroxidase (GPx) (immunoenzyme technique). Statistical analysis was performed using Mann-Whitney U test. The neonates of diabetic rats were smaller than healthy ones and serum glucose concentration was markedly higher in diabetic animals, especially in model I. MDA levels increased significantly, whereas GSH content and SOD as well as GPx activities were markedly diminished in diabetic pregnant rats and their offspring in comparison with the control group. In animals supplemented with tocopherol MDA concentrations declined significantly, GSH content and SOD activities were markedly elevated in almost all types of tissues studied, whereas glutathione peroxidase remained suppressed.
By monitoring the activity of chosen scavenging enzymes in homogenates and body fluids we can accumulate information on ongoing biological oxidative stress. Our results suggest that diabetic pregnant rats and their neonates are exposed to an increased oxidative stress, but vitamin E supplementation could at least in part reduce its detrimental effects.
我们研究的目的是确定中等剂量的非酶抗氧化剂维生素E是否能在妊娠糖尿病大鼠及其后代的抗氧化防御系统中发挥作用。使用了两种实验性糖尿病模型：第一组由30只雌性Wistar大鼠组成，给它们单次注射链脲佐菌素（40毫克/千克），7天后进行交配，而第二组在妊娠第7天诱导糖尿病（使用相同剂量的链脲佐菌素）。在实验的每个部分，糖尿病动物被分成两个相等的组：补充维生素E组和仅喂食标准饮食组。我们的对照组由20只正常大鼠组成。分娩后1天，将大鼠断头，制备母体肝脏和子宫以及新生大鼠肺和肝脏的匀浆。然后测量以下参数：匀浆和血清中丙二醛（MDA）浓度（比色法）、谷胱甘肽（GSH）水平（比色试验）、铜锌超氧化物歧化酶（SOD）活性（分光光度法）和谷胱甘肽过氧化物酶（GPx）活性（免疫酶技术）。使用曼-惠特尼U检验进行统计分析。糖尿病大鼠的新生仔比健康仔小，糖尿病动物的血清葡萄糖浓度明显更高，尤其是在模型I中。与对照组相比，糖尿病妊娠大鼠及其后代的MDA水平显著升高，而GSH含量以及SOD和GPx活性明显降低。在补充生育酚的动物中，MDA浓度显著下降，几乎所有研究的组织类型中GSH含量和SOD活性明显升高，而谷胱甘肽过氧化物酶仍然受到抑制。
通过监测匀浆和体液中选定清除酶的活性，我们可以积累有关正在进行的生物氧化应激的信息。我们的结果表明，糖尿病妊娠大鼠及其新生仔暴露于增加的氧化应激中，但补充维生素E至少可以部分降低其有害影响。
