口服鸡矢藤提取物后大鼠血浆中环烯醚萜苷及其代谢产物的超高效液相色谱/四极杆飞行时间质谱分析

UPLC/Q-TOF-MS analysis of iridoid glycosides and metabolites in rat plasma after oral administration of Paederia scandens extracts.

作者信息

Wang Dong-Mei, Xu Yi-Fei, Chen Zhu, Huang Lin-Fang, Chen Shi-Lin

机构信息

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.

Department of pharmacy, Institute of pharmacy, Guangzhou Traditional Chinese Medicine University, Guangzhou 510006, China.

出版信息

Chin J Nat Med. 2015 Mar;13(3):215-21. doi: 10.1016/S1875-5364(15)30007-8.

DOI:10.1016/S1875-5364(15)30007-8

PMID:25835366

Abstract

A rapid and validated UPLC-MS method was developed for investigating the absorbed components of Paederia scandens (Lour.) Merrill (P. scandensy) in rat plasma. The bioactive constituents in plasma samples from rats administrated orally with P. scandens extract were analyzed by Ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Four prototype compounds were identified in rat serum as potential bioactive components of P. scandens by comparing their retention times and mass spectrometry data or by mass spectrometry analysis and retrieving the reference literatures. Glucuronidation after deglycosylation was the major metabolic pathway for the iridoid glycosides in P. scandens. These results showed that the methods had high sensitivity and resolution and were suitable for identifying the bioactive constituents in plasma after oral administration of P. scandens. providing helpful chemical information for further pharmacological and mechanistic researched on the P. scandens.

摘要

建立了一种快速且经过验证的超高效液相色谱-质谱（UPLC-MS）方法，用于研究鸡矢藤在大鼠血浆中的吸收成分。采用超高效液相色谱四极杆飞行时间质谱（UPLC-Q-TOF-MS）分析口服鸡矢藤提取物的大鼠血浆样本中的生物活性成分。通过比较其保留时间和质谱数据，或通过质谱分析并检索参考文献，在大鼠血清中鉴定出四种原型化合物作为鸡矢藤的潜在生物活性成分。去糖基化后的葡萄糖醛酸化是鸡矢藤中环烯醚萜苷的主要代谢途径。这些结果表明，该方法具有高灵敏度和分辨率，适用于鉴定口服鸡矢藤后血浆中的生物活性成分，为鸡矢藤的进一步药理和作用机制研究提供了有用的化学信息。