用于再生医学的纤维蛋白水凝胶，以递送根尖乳头的牙干细胞。
Fibrin hydrogels to deliver dental stem cells of the apical papilla for regenerative medicine.
作者信息
Germain Loïc, De Berdt Pauline, Vanacker Julie, Leprince Julian, Diogenes Anibal, Jacobs Damien, Vandermeulen Gaëlle, Bouzin Caroline, Préat Véronique, Dupont-Gillain Christine, des Rieux Anne
机构信息
Department of Advanced Drug Delivery & Biomaterials, Louvain Drug Research Institute, Université Catholique de Louvain, Brussels, Belgium.
出版信息
Regen Med. 2015;10(2):153-67. doi: 10.2217/rme.14.81.
AIM
Evaluation of survival, proliferation and neurodifferentiation of dental stem cells from the apical papilla (SCAP) in fibrin hydrogels. We hypothesized that fibrin composition will influence cell behavior.
METHODS
Modulus, pore and fiber size were measured. SCAP in vitro viability, proliferation and neural differentiation, as well as in vivo proliferation and angiogenesis were studied.
RESULTS
Hydrogel moduli were influenced by fibrin formulation but not hydrogel morphology, SCAP in vitro viability and proliferation. In total 60% of SCAP expressed PanNeurofilament in vitro without induction in Fibrinogen50-Thrombin10. SCAP proliferated when implanted in vivo and stimulated host endothelial cell infiltration.
CONCLUSION
Fibrinogen30-Thrombin10 or Thrombin50 would be more favorable to in vitro SCAP viability and in vivo proliferation, while Fibrinogen 50-Thrombin50 would be more adapted to neurodifferentiation.
目的
评估根尖乳头干细胞（SCAP）在纤维蛋白水凝胶中的存活、增殖及神经分化情况。我们假设纤维蛋白成分会影响细胞行为。
方法
测量模量、孔径和纤维大小。研究了SCAP的体外活力、增殖和神经分化，以及体内增殖和血管生成情况。
结果
水凝胶模量受纤维蛋白配方影响，但不受水凝胶形态、SCAP体外活力和增殖的影响。在纤维蛋白原50-凝血酶10中，总共60%的SCAP在体外未诱导的情况下表达泛神经丝蛋白。SCAP植入体内后增殖，并刺激宿主内皮细胞浸润。
结论
纤维蛋白原30-凝血酶10或凝血酶50对体外SCAP活力和体内增殖更有利，而纤维蛋白原50-凝血酶50更适合神经分化。
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