Huang W, Hasegawa T, Imai Y, Takeda D, Akashi M, Komori T
Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
Int J Oral Maxillofac Surg. 2015 Jul;44(7):929-35. doi: 10.1016/j.ijom.2015.03.001. Epub 2015 Mar 30.
We previously demonstrated that human mandibular fracture haematoma-derived cells (MHCs) play an important role in mandibular fracture healing and that low-intensity pulsed ultrasound (LIPUS) accelerates this effect by stimulating various osteogenic cytokines. In the present study, we investigated how LIPUS affects the expression of bone morphogenetic proteins (BMPs), which are also known to have the ability to induce bone formation. MHCs were isolated from human mandibular fracture haematomas and the cells were divided into two groups: a LIPUS (+) group and a LIPUS (-) group, both of which were cultured in osteogenic medium. LIPUS was applied to the LIPUS (+) group 20 min a day for 4, 8, 14, and 20 days (1.5 MHz, 30 mW/cm(2)). Real-time PCR and immunofluorescence studies were carried out to determine the expression of BMP-2, 4, and 7. Compared to the LIPUS (-) group, gene expression levels were significantly increased in the LIPUS (+) group for BMP-2 on day 20 (67.38 ± 26.59 vs. 11.52 ± 3.42, P < 0.001), for BMP-4 on days 14 (45.12 ± 11.06 vs. 9.20 ± 2.88, P = 0.045) and 20 (40.96 ± 24.81 vs. 3.22 ± 1.53, P = 0.035), and for BMP-7 on day 8 (48.11 ± 35.36 vs. 7.03 ± 3.96, P = 0.034). These findings suggest that BMP-2, 4, and 7 may be mediated by LIPUS therapy during the bone repair process.
我们之前证实，人下颌骨骨折血肿来源细胞（MHCs）在颌骨骨折愈合中发挥重要作用，且低强度脉冲超声（LIPUS）通过刺激多种成骨细胞因子来加速这一作用。在本研究中，我们探究了LIPUS如何影响骨形态发生蛋白（BMPs）的表达，已知BMPs也具有诱导骨形成的能力。从人下颌骨骨折血肿中分离出MHCs，并将细胞分为两组：LIPUS（+）组和LIPUS（-）组，两组均在成骨培养基中培养。对LIPUS（+）组每天施加LIPUS 20分钟，持续4、8、14和20天（1.5兆赫，30毫瓦/平方厘米）。进行实时聚合酶链反应（PCR）和免疫荧光研究以确定BMP - 2、4和7的表达。与LIPUS（-）组相比，LIPUS（+）组在第20天时BMP - 2的基因表达水平显著升高（67.38±26.59对11.52±3.42，P<0.001），在第14天和第20天时BMP - 4的基因表达水平显著升高（第14天：45.12±11.06对9.20±2.88，P = 0.045；第20天：40.96±24.81对3.22±1.53，P = 0.035），在第8天时BMP - 7的基因表达水平显著升高（48.11±35.36对7.03±3.96，P = 0.034）。这些发现表明，在骨修复过程中，BMP - 2、4和7可能由LIPUS疗法介导。
