Hasegawa T, Miwa M, Sakai Y, Niikura T, Kurosaka M, Komori T
Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, Hyogo 650-0017, Japan.
J Bone Joint Surg Br. 2009 Feb;91(2):264-70. doi: 10.1302/0301-620X.91B2.20827.
The haematoma occurring at the site of a fracture is known to play an important role in bone healing. We have recently shown the presence of progenitor cells in human fracture haematoma and demonstrated that they have the capacity for multilineage mesenchymal differentiation. There have been many studies which have shown that low-intensity pulsed ultrasound (LIPUS) stimulates the differentiation of a variety of cells, but none has investigated the effects of LIPUS on cells derived from human fracture tissue including human fracture haematoma-derived progenitor cells (HCs). In this in vitro study, we investigated the effects of LIPUS on the osteogenic activity of HCs. Alkaline phosphatase activity, osteocalcin secretion, the expression of osteoblast-related genes and the mineralisation of HCs were shown to be significantly higher when LIPUS had been applied but without a change in the proliferation of the HCs. These findings provide evidence in favour of the use of LIPUS in the treatment of fractures.
众所周知，骨折部位出现的血肿在骨愈合过程中起着重要作用。我们最近发现人类骨折血肿中存在祖细胞，并证明它们具有多谱系间充质分化的能力。已有许多研究表明，低强度脉冲超声（LIPUS）能刺激多种细胞的分化，但尚无研究探讨LIPUS对源自人类骨折组织的细胞（包括人类骨折血肿衍生祖细胞（HCs））的影响。在这项体外研究中，我们研究了LIPUS对HCs成骨活性的影响。结果显示，施加LIPUS后，HCs的碱性磷酸酶活性、骨钙素分泌、成骨细胞相关基因的表达以及矿化均显著升高，但HCs的增殖未发生变化。这些发现为LIPUS用于骨折治疗提供了证据支持。
