Zeng Guang, Shen Huan, Tang Guo, Cai Xingwei, Bi Lixia, Sun Bin, Yang Yongjin, Xun Wenxing
Department of Plastic and Burn Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an, 710038, People's Republic of China.
Department of Stomatology, The General Hospital of the Second Artillery Corps of Chinese PLA, Beijing, 100088, People's Republic of China.
Tumour Biol. 2015 Sep;36(9):6781-8. doi: 10.1007/s13277-015-3359-5. Epub 2015 Apr 3.
In the present study, we isolated and characterized a homogenous polysaccharide (GIAP1) from the alkaline extract of the roots of Glycyrrhiza inflata. The anti-tumor activity of GIAP1 toward human oral cancer SCC-25 cells and the underlying mechanisms were also examined in vitro. GIAP1 dose-dependently inhibited the proliferation of SCC-25 cells via inducing apoptosis. Moreover, GIAP1 downregulated Bax/Bcl-2 ratio, disrupted the mitochondrial membrane potential (MMP), and caused the release of cytochrome c to cytosol. Besides, GIAP1 triggered activation of capase-3 and caspase-9, as well as the degradation of poly (ADP-ribose) polymerase (PARP). In addition, the caspase-3 or caspase-9 inhibitor significantly inhibited GIAP1-induced apoptosis in SCC-25 cells. Collectively, we can conclude that the GIAP1 induces apoptosis in SCC-25 cells via a mitochondrial pathway.
在本研究中，我们从胀果甘草根的碱性提取物中分离并鉴定了一种同质多糖（GIAP1）。还在体外检测了GIAP1对人口腔癌SCC - 25细胞的抗肿瘤活性及其潜在机制。GIAP1通过诱导凋亡剂量依赖性地抑制SCC - 25细胞的增殖。此外，GIAP1下调Bax/Bcl - 2比值，破坏线粒体膜电位（MMP），并导致细胞色素c释放到细胞质中。此外，GIAP1触发了caspase - 3和caspase - 9的激活，以及聚（ADP - 核糖）聚合酶（PARP）的降解。此外，caspase - 3或caspase - 9抑制剂显著抑制GIAP1诱导的SCC - 25细胞凋亡。总体而言，我们可以得出结论，GIAP1通过线粒体途径诱导SCC - 25细胞凋亡。
