Department of Otolaryngology Head and Neck Surgery, First Affiliated Hospital of Chinese PLA General Hospital, Beijing 100048, China.
Carbohydr Polym. 2013 Feb 15;92(2):1040-5. doi: 10.1016/j.carbpol.2012.10.024. Epub 2012 Oct 18.
One water-soluble polysaccharide fraction (WACP) was purified from the Artemisia capillaris by DEAE-cellulose anion-exchange and Sephacryl S-400 gel-permeation chromatography. Based on gas chromatography (GC) analysis, WACP was an arabinogalactan (AG), consisting of arabinose and galactose in the ratio of 4:2. Its molecular weight was about 5.8×10(4) Da. The present study demonstrated the anticancer potential of WACP on human nasopharyngeal carcinoma CNE-2 cells and elucidated its possible mechanisms. MTT and flow cytometric analysis identified that WACP had a potent anti-proliferation activity on CNE-2 cells by inducing apoptosis, which was parallel with the morphological changes of CNE-2 cells under inverted microscope. In addition, WACP triggered the apoptosis via the mitochondrial apoptotic pathway, which included the loss of mitochondrial membrane potential, release of cytochrome c and activation of caspase-3/9. Taken together, these results suggest that WACP has anticancer potential in the treatment of human nasopharyngeal carcinoma.
一种水溶性多糖（WACP）通过 DEAE-纤维素阴离子交换和 Sephacryl S-400 凝胶渗透色谱从青蒿中纯化。基于气相色谱（GC）分析，WACP 是一种阿拉伯半乳聚糖（AG），由阿拉伯糖和半乳糖以 4:2 的比例组成。其分子量约为 5.8×10(4)Da。本研究证明了 WACP 对人鼻咽癌 CNE-2 细胞的抗癌潜力，并阐明了其可能的机制。MTT 和流式细胞术分析表明，WACP 通过诱导细胞凋亡对 CNE-2 细胞具有很强的增殖抑制活性，这与倒置显微镜下 CNE-2 细胞的形态变化一致。此外，WACP 通过线粒体凋亡途径触发细胞凋亡，其中包括线粒体膜电位丧失、细胞色素 c 释放和 caspase-3/9 的激活。总之，这些结果表明 WACP 在治疗人鼻咽癌方面具有抗癌潜力。
