双锥形光纤探针用于快速无标记免疫分析。

Biconically tapered fiber optic probes for rapid label-free immunoassays.

机构信息

Department of Physics and Astronomy, University of California, Los Angeles, 475 Portola Plaza, Los Angeles, CA 90095, USA.

Department of Plant and Microbial Biology, University of California, Berkeley, 565 Li Ka Shing Center, Berkeley, CA 94720, USA.

出版信息

Biosensors (Basel). 2015 Apr 1;5(2):158-71. doi: 10.3390/bios5020158.

DOI:10.3390/bios5020158

PMID:25836359

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4493543/

Abstract

We report use of U-shaped biconically tapered optical fibers (BTOF) as probes for label-free immunoassays. The tapered regions of the sensors were functionalized by immobilization of immunoglobulin-G (Ig-G) and tested for detection of anti-IgG at concentrations of 50 ng/mL to 50 µg/mL. Antibody-antigen reaction creates a biological nanolayer modifying the waveguide structure leading to a change in the sensor signal, which allows real-time monitoring. The kinetics of the antibody (mouse Ig-G)-antigen (rabbit anti-mouse IgG) reactions was studied. Hydrofluoric acid treatment makes the sensitive region thinner to enhance sensitivity, which we confirmed by experiments and simulations. The limit of detection for the sensor was estimated to be less than 50 ng/mL. Utilization of the rate of the sensor peak shift within the first few minutes of the antibody-antigen reaction is proposed as a rapid protein detection method.

摘要

我们报告了使用 U 型双锥形光纤（BTOF）作为无标记免疫分析的探头。传感器的锥形区域通过固定免疫球蛋白 G（Ig-G）进行功能化，并测试了其在 50ng/mL 至 50μg/mL 浓度下检测抗 Ig-G 的能力。抗体-抗原反应会产生生物纳米层，从而改变波导结构，导致传感器信号发生变化，从而实现实时监测。研究了抗体（鼠 Ig-G）-抗原（兔抗鼠 IgG）反应的动力学。氢氟酸处理使敏感区域变薄，从而提高了灵敏度，我们通过实验和模拟进行了验证。传感器的检测限估计小于 50ng/mL。我们提出利用抗体-抗原反应最初几分钟内传感器峰值移动的速率作为一种快速蛋白质检测方法。