Zhang Yan, Shibru Helen, Cooper Kristie L, Wang Anbo
Center for Photonics Technology, Bradley Department of Electrical and Computer Engineering, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061, USA.
Opt Lett. 2005 May 1;30(9):1021-3. doi: 10.1364/ol.30.001021.
A fiber-optic sensor is designed based on multicavity Fabry-Perot interferometry for the study of optical thickness in self-assembled thin-film layers. This miniature sensor is applicable not only to the measurement of self-assembled polyelectrolyte layers but also to the immobilization of proteins such as immunoglobulin G (IgG). The binding of IgG and the corresponding antigen is observed, and the nonspecific binding characteristics are investigated. The optical thickness changes are used to evaluate the immobilization of the IgG and the immunological activities of the immobilized layers.
基于多腔法布里-珀罗干涉测量技术设计了一种光纤传感器，用于研究自组装薄膜层的光学厚度。这种微型传感器不仅适用于自组装聚电解质层的测量，还适用于蛋白质（如免疫球蛋白G，IgG）的固定化。观察了IgG与相应抗原的结合情况，并研究了非特异性结合特性。利用光学厚度变化来评估IgG的固定化情况以及固定化层的免疫活性。
