慢性丁型肝炎：通过免疫印迹法检测血清中的丁型肝炎病毒抗原及其与丁型病毒复制其他标志物的相关性

Chronic delta hepatitis: detection of hepatitis delta virus antigen in serum by immunoblot and correlation with other markers of delta viral replication.

作者信息

Buti M, Esteban R, Jardi R, Rodriguez-Frias F, Casacuberta J, Esteban J I, Allende E, Guardia J

机构信息

Department of Internal Medicine, Hospital General Vall D'Hebro, Universidad Autonoma, Barcelona, Spain.

出版信息

Hepatology. 1989 Dec;10(6):907-10. doi: 10.1002/hep.1840100602.

DOI:10.1002/hep.1840100602

PMID:2583685

Abstract

To investigate the presence of serum hepatitis delta virus antigen by immunoblot and its correlation with other markers of active viral replication (intrahepatic hepatitis D antigen, IgM antibody to hepatitis D and serum hepatitis D virus RNA), we studied serum samples from 50 patients with chronic hepatitis D virus infection (38 with and 12 without intrahepatic hepatitis D antigen). Of the 38 patients with intrahepatic hepatitis D antigen, 27 (71%) had antigen detectable in serum by immunoblot, whereas only two were reactive by conventional enzyme-linked immunosorbent assay. Thirty-one (82%) patients were also positive for serum hepatitis D virus RNA by spot hybridization and 33 (87%) were positive for IgM anti-hepatitis D virus. All markers were simultaneously present in 24 patients. Circulating hepatitis D antigen was detected in one (8%), IgM anti-hepatitis D in seven (58%) and hepatitis D virus RNA in two (17%) of the 12 patients who had anti-hepatitis D in serum but not detectable hepatitis D antigen in liver. Hepatitis D antigen was not detected in serum of any of the 15 control patients. The results suggest that serum hepatitis D antigen as detected by immunoblot and serum hepatitis D virus RNA are similar in sensitivity for detection of active hepatitis D virus replication during chronic infection and constitute useful, sensitive and noninvasive tests for the diagnosis and monitoring of chronic hepatitis D virus infection.

摘要

为通过免疫印迹法研究血清中丁型肝炎病毒抗原的存在情况及其与其他病毒活跃复制标志物（肝内丁型肝炎抗原、抗丁型肝炎 IgM 抗体和血清丁型肝炎病毒 RNA）的相关性，我们研究了 50 例慢性丁型肝炎病毒感染患者的血清样本（38 例有肝内丁型肝炎抗原，12 例无肝内丁型肝炎抗原）。在 38 例有肝内丁型肝炎抗原的患者中，27 例（71%）通过免疫印迹法可在血清中检测到抗原，而通过传统酶联免疫吸附试验仅有 2 例呈反应性。31 例（82%）患者的血清丁型肝炎病毒 RNA 通过斑点杂交呈阳性，33 例（87%）抗丁型肝炎病毒 IgM 呈阳性。所有标志物同时存在于 24 例患者中。在血清中有抗丁型肝炎但肝内未检测到丁型肝炎抗原的 12 例患者中，1 例（8%）检测到循环丁型肝炎抗原，7 例（58%）检测到抗丁型肝炎病毒 IgM，2 例（17%）检测到丁型肝炎病毒 RNA。15 例对照患者的血清中均未检测到丁型肝炎抗原。结果表明，免疫印迹法检测的血清丁型肝炎抗原和血清丁型肝炎病毒 RNA 在慢性感染期间检测丁型肝炎病毒活跃复制的敏感性相似，是诊断和监测慢性丁型肝炎病毒感染的有用、敏感且非侵入性的检测方法。