Simpson L H, Battegay M, Hoofnagle J H, Waggoner J G, Di Bisceglie A M
Liver Diseases Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Dig Dis Sci. 1994 Dec;39(12):2650-5. doi: 10.1007/BF02087704.
We studied 28 patients with chronic delta hepatitis for the presence of hepatitis delta virus (HDV) RNA in serum. The hot start polymerase chain reaction (PCR) method, in which the reaction begins at 60-80 degrees C, showed a higher sensitivity than conventional PCR reaction. Additionally, the presence of hepatitis B (HBV) and C virus (HCV) infections were determined by PCR. HDV RNA was detected in 26 patients (93%), HBV DNA in 22 (79%), and HCV RNA in only one. Detection of HDV RNA correlated very well with detection of hepatitis delta antigen by immunostaining in the liver. In six patients HDV RNA was detectable despite the absence of HBV DNA in serum, suggesting that high levels of HBV are not required for HDV replication. Of 29 control patients with chronic hepatitis B without antibody to HDV, none had detectable HDV RNA, while all had HBV DNA in serum. Detection of HDV RNA with PCR proved highly sensitive and specific, demonstrating that virtually all patients with chronic HDV infection had ongoing viral replication.
我们研究了28例慢性丁型肝炎患者血清中丁型肝炎病毒（HDV）RNA的存在情况。热启动聚合酶链反应（PCR）方法，即反应在60 - 80摄氏度开始，显示出比传统PCR反应更高的灵敏度。此外，通过PCR检测乙型肝炎（HBV）和丙型肝炎病毒（HCV）感染情况。在26例患者（93%）中检测到HDV RNA，22例（79%）中检测到HBV DNA，仅1例中检测到HCV RNA。HDV RNA的检测与肝脏中通过免疫染色检测丁型肝炎抗原的结果非常吻合。在6例血清中无HBV DNA但仍可检测到HDV RNA的患者中，提示HDV复制不需要高水平的HBV。在29例无HDV抗体的慢性乙型肝炎对照患者中，无一例检测到HDV RNA，而所有患者血清中均有HBV DNA。用PCR检测HDV RNA证明具有高度的敏感性和特异性，表明几乎所有慢性HDV感染患者都有持续的病毒复制。
