Yan Wenjin, Hou Beiwei, Xue Qingyun, Geng Lixia, Ding Xiaoyu
College of Life Sciences, Nanjing Normal University, No. 1, Wenyuan Road, Nanjing, 210046, China.
Genetica. 2015 Jun;143(3):361-71. doi: 10.1007/s10709-015-9835-4. Epub 2015 Apr 3.
Knowledge of population genetic structure and intrapopulation genetic variation is important for understanding population dynamics and evolutionary processes. Dendrobium nobile is an endangered traditional Chinese tonic medicine. In order to analyze the population differentiation and genetic diversity in D. nobile and propose proper conservation measurements, we genotyped 102 individual plants from 7 natural populations distributed across southwest China at 9 microsatellite loci. Seven pairs of genomic SSR primers were newly designed, and two pairs were chosen from the EST-SSRs. According to the results, the genetic process of D. nobile on Hainan Island and the Chinese mainland might be affected by different evolutionary processes, the genetic drift caused by founder effect has played an important role in shaping the genetic constitution of the Island population. The population of D. nobile in Hainan Island was highly differentiated and displayed low levels of genetic diversity. For the conservation management plans of D. nobile, we propose that individuals in Hainan Island with rare alleles need to be conserved with top priority, and those individuals with rare alleles and the most common alleles also should be concerned. The seven new microsatellite loci may be informative for further evaluation and conservation of the genetic diversity of D. nobile.
了解种群遗传结构和种群内遗传变异对于理解种群动态和进化过程至关重要。铁皮石斛是一种濒危的传统中药材。为了分析铁皮石斛的种群分化和遗传多样性并提出适当的保护措施，我们对来自中国西南地区7个自然种群的102株个体在9个微卫星位点进行了基因分型。新设计了7对基因组SSR引物，从EST-SSRs中选取了2对。结果表明，海南岛和中国大陆铁皮石斛的遗传过程可能受到不同进化过程的影响，奠基者效应引起的遗传漂变在塑造岛屿种群的遗传构成中发挥了重要作用。海南岛的铁皮石斛种群高度分化，遗传多样性水平较低。对于铁皮石斛的保护管理计划，我们建议优先保护海南岛具有稀有等位基因的个体，同时也应关注那些具有稀有等位基因和最常见等位基因的个体。这7个新的微卫星位点可能有助于进一步评估和保护铁皮石斛的遗传多样性。
