Plant Biotechnology Laboratory, Department of Botany, Centre for Advanced Studies, North-Eastern Hill University, Shillong 793022, Meghalaya, India.
Gene. 2013 Oct 15;529(1):21-6. doi: 10.1016/j.gene.2013.07.096. Epub 2013 Aug 11.
Genetic variability in the wild genotypes of Dendrobium nobile Lindl. collected from different parts of Northeast India, was analyzed using a Start Codon Targeted (SCoT) marker system. A total of sixty individuals comprising of six natural populations were investigated for the existing natural genetic diversity. One hundred and thirty two (132) amplicons were produced by SCoT marker generating 96.21% polymorphism. The PIC value of the SCoT marker system was 0.78 and the Rp values of the primers ranged between 4.43 and 7.50. The percentage of polymorphic loci (Pp) ranging from 25% to 56.82%, Nei's gene diversity (h) from 0.08 to 0.15 with mean Nei's gene diversity of 0.28, and Shannon's information index (I) values ranging from 0.13 to 0.24 with an average value of 0.43 were recorded. The gene flow value (0.37) and the diversity among populations (0.57) demonstrated higher genetic variation among the populations. Analysis of molecular variance (AMOVA) showed 43.37% of variation within the populations, whereas 56.63% variation was recorded among the populations. Cluster analysis also reveals high genetic variation among the genotypes. Present investigation suggests the effectiveness of SCoT marker system to estimate the genetic diversity of D. nobile and that it can be seen as a preliminary point for future research on the population and evolutionary genetics of this endangered orchid species of medicinal importance.
采用起始密码子目标（SCoT）标记系统分析了从印度东北部不同地区采集的野生铁皮石斛（Dendrobium nobile Lindl.）野生基因型的遗传变异。对来自六个自然种群的 60 个个体进行了现有的天然遗传多样性研究。SCoT 标记产生了 132 个扩增子，产生了 96.21%的多态性。SCoT 标记系统的 PIC 值为 0.78，引物的 Rp 值在 4.43 到 7.50 之间。多态性位点（Pp）的百分比范围为 25%至 56.82%，Nei 基因多样性（h）从 0.08 至 0.15，平均 Nei 基因多样性为 0.28，Shannon 信息指数（I）值范围为 0.13 至 0.24，平均值为 0.43。基因流值（0.37）和种群间的多样性（0.57）表明种群间存在较高的遗传变异。分子方差分析（AMOVA）显示，种群内有 43.37%的变异，而种群间有 56.63%的变异。聚类分析也揭示了基因型之间的高遗传变异。目前的研究表明，SCoT 标记系统可用于评估铁皮石斛的遗传多样性，并且可以作为该具有药用价值的濒危兰花种的种群和进化遗传学未来研究的初步切入点。
