van Westen Els, Wijmenga-Monsuur Alienke J, van Dijken Harry H, van Gaans-van den Brink Jacqueline A M, Kuipers Betsy, Knol Mirjam J, Berbers Guy A M, Sanders Elisabeth A M, Rots Nynke Y, van Els Cécile A C M
Centre for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven.
Centre for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven Department of Pediatric Immunology and Infectious Diseases, Wilhelmina Children's Hospital, University Medical Center, Utrecht, The Netherlands.
Clin Infect Dis. 2015 Aug 1;61(3):342-9. doi: 10.1093/cid/civ274. Epub 2015 Apr 1.
Both the 10- and 13-valent pneumococcal conjugate vaccines (PCV10 and PCV13) induce immunological memory against Streptococcus pneumoniae infections caused by vaccine serotypes. In addition to comparing serum antibody levels, we investigated frequencies of serotype-specific plasma cells (PCs) and memory B-cells (Bmems) as potential predictors of long-term immunity around the booster vaccination at 11 months of age.
Infants were immunized with PCV10 or PCV13 at 2, 3, 4, and 11 months of age. Blood was collected before the 11-month booster or 7-9 days afterward. Serotype-specific immunoglobulin G (IgG) levels were determined in serum samples by multiplex immunoassay. Circulating specific PCs and Bmems against shared serotypes 1, 6B, 7F, and 19F and against PCV13 serotypes 6A and 19A were measured in peripheral blood mononuclear cells by enzyme-linked immunospot assay.
No major differences in IgG levels and PC frequencies between groups were found for the 4 shared serotypes. Notably, PCV13 vaccination resulted in higher frequencies of Bmems than PCV10 vaccination, both before and after the booster dose, for all 4 shared serotypes except for serotype 1 postbooster. For PCV13-specific serotypes 6A and 19A, the IgG levels and frequencies of PCs and Bmems were higher in the PCV13 group, pre- and postbooster, except for PC frequencies prebooster.
Both PCVs are immunogenic and induce measurable IgG, PC, and Bmem booster responses at 11 months. Compared to PCV10, vaccination with PCV13 was associated with overall similar IgG levels and PC frequencies but with higher Bmem frequencies before and after the 11-month booster. The clinical implications of these results need further follow-up.
NTR3069.
10价和13价肺炎球菌结合疫苗（PCV10和PCV13）均可诱导针对疫苗血清型引起的肺炎链球菌感染的免疫记忆。除了比较血清抗体水平外，我们还研究了血清型特异性浆细胞（PC）和记忆B细胞（Bmems）的频率，作为11月龄加强免疫前后长期免疫的潜在预测指标。
婴儿在2、3、4和11月龄时接种PCV10或PCV13。在11月龄加强免疫前或之后7 - 9天采集血液。通过多重免疫测定法测定血清样本中的血清型特异性免疫球蛋白G（IgG）水平。通过酶联免疫斑点测定法在外周血单个核细胞中测量针对共同血清型1、6B、7F和19F以及针对PCV13血清型6A和19A的循环特异性PC和Bmems。
对于4种共同血清型，两组之间的IgG水平和PC频率没有发现主要差异。值得注意的是，对于所有4种共同血清型（加强免疫后血清型1除外），PCV13接种在加强免疫前后均导致Bmems频率高于PCV10接种。对于PCV13特异性血清型6A和19A，PCV13组在加强免疫前后的IgG水平、PC和Bmems频率均较高，但加强免疫前PC频率除外。
两种PCV均具有免疫原性，并在11月龄时诱导可测量的IgG、PC和Bmem加强反应。与PCV10相比，PCV13接种与总体相似的IgG水平和PC频率相关，但在11月龄加强免疫前后Bmem频率更高。这些结果的临床意义需要进一步随访。
NTR3069。
